{"product_id":"abcam-ab167713","title":"Abcam, ab167713, Recombinant human PD-L1 protein (Active)","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant human PD-L1 protein (Active) is a Human Fragment protein, in the 19 to 238 aa range, expressed in HEK 293 cells, with \u0026gt;98%, \u0026lt; 1 EU\/µg endotoxin level, suitable for SDS-PAGE, HPLC, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;98% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, HPLC, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Immobilized ab167713 on Anti-His Tag (C-term) Antibody, precoated (0.1 μg\/well) plate, binds Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified), at 5 μg\/mL (100 μL\/well) with a linear range of 10-78 ng\/mL.,\u003cbr\u003e\nAccession:Q9NZQ7,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute with sterile deionized water to a concentration of 400 µg\/ml.,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: PBS, 5% Trehalose\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nFor a recombinant rabbit monoclonal antibody to PD-L1 in IHC usage and KO validated,\u003cbr\u003e\nab205921\u003cbr\u003e\n(clone 28-8)\u003cbr\u003e\nFor a recombinant rabbit monoclonal antibody to PD-L1 in WB and ICC\/IF usage, -\u003cbr\u003e\nab174838\u003cbr\u003e\n(clone EPR1161(2))\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPD-L1 also known as Programmed Death-Ligand 1 or CD274 is a protein involved in immune modulation. Mechanically PD-L1 interacts with its receptors particularly PD-1 to regulate cellular immune responses. This transmembrane protein has a calculated molecular weight of approximately 33 kDa. PD-L1 is expressed on various cell types including tumor cells and immune cells such as dendritic cells macrophages and B cells. Its expression is often upregulated in response to inflammatory cytokines.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPD-L1 plays a central role in immune evasion mechanisms utilized by tumors. It is not part of a larger protein complex but directly interacts with PD-1 and CD80. When PD-L1 binds to PD-1 it sends inhibitory signals leading to decreased T cell activation and proliferation allowing cancer cells to avoid immune destruction. PD-L1 expression provides a mechanism for tumors to suppress immune surveillance facilitating tumor progression.\u003cbr\u003e\nPathways\u003cbr\u003e\nPD-L1 is integral to the immune checkpoint pathway which is an important regulator of immune response. The interaction between PD-L1 and PD-1 provides a mechanism for immune tolerance which is part of the broader adaptive immune system pathway. PD-L1 is related to other immune checkpoint proteins such as CTLA-4 in its function to limit autoreactivity and promote immune homeostasis.\u003cbr\u003e\nPD-L1 is most associated with cancer particularly in tumors such as melanoma and non-small cell lung cancer. PD-L1 expression on tumor cells often correlates with poor prognosis. PD-L1 directly interacts with PD-1 in these cancers a target for immunotherapies such as checkpoint inhibitors which aim to block this interaction and restore immune activity against tumors. PD-L1 involvement extends to autoimmune disorders where altered expression can impact tolerance and lead to immune-related tissue damage.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843809792169,"sku":"ab167713","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab167713","provider":"Iright","version":"1.0","type":"link"}