{"product_id":"abcam-ab169540","title":"Abcam, ab169540, Anti-Ndufs1 antibody [EPR11521(B)]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Ndufs1 antibody [EPR11521(B)] (ab169540) is a rabbit monoclonal antibody detecting Ndufs1 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR11521(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, IP, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-Ndufs1 antibody [EPR11521(B)] (ab169540) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of Ndufs1?\u003cbr\u003e\nAnti-Ndufs1 [EPR11521(B)] (ab169540) specifically detects a band for Ndufs1 (UniProt: P28331) at a molecular weight of 79kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-Ndufs1 [EPR11521(B)] (ab169540) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR11521(B)] also available for your convenience: ab169540, Alexa Fluor® 488 -\u003cbr\u003e\nab198954\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab198956\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab249492\u003cbr\u003e\n, PE -\u003cbr\u003e\nab305910\u003cbr\u003e\n, APC -\u003cbr\u003e\nab305911\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab305912\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308883\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310609\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab312137\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312623\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321162\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNdufs1 known as NADH:ubiquinone oxidoreductase core subunit S1 or complex I 75 kDa subunit forms a significant part of the mitochondrial electron transport chain. This protein shows a mass of 75 kDa and is primarily found in mitochondria among various tissues. Ndufs1 plays an important role in transferring electrons from NADH to ubiquinone facilitating proton translocation across the inner mitochondrial membrane. With its function it contributes to the creation of a proton gradient essential for ATP synthesis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNdufs1 facilitates the primary catalytic activity of the NADH dehydrogenase complex also found within the mitochondrial complex I. By being part of this large multi-subunit complex it helps in electron flow which essential for cellular respiration. Proper functioning of Ndufs1 becomes necessary for maintaining adequate energy production through ATP synthesis in cells impacting energy-dependent processes significantly.\u003cbr\u003e\nPathways\u003cbr\u003e\nNdufs1 acts mainly within the oxidative phosphorylation pathway. This pathway is indispensable for energy conversion in cells prominently featuring NADH dehydrogenase (ubiquinone). It relates closely with other critical proteins like Ndufa9 and Ndufb8 as these proteins also integrate into the mitochondrial complex I ensuring effective electron transport and energy yield in cells. The pathway links metabolic flux to energy production keeping cellular operations efficient.\u003cbr\u003e\nNdufs1 mutations correlate with mitochondrial diseases such as Leigh syndrome and mitochondrial complex I deficiency. These conditions often manifest neurological and muscular symptoms due to impaired mitochondrial function. Ndufs1 connections with proteins like Ndufv1 and additional complex I subunits emphasize its role in regular mitochondrial activity making its study relevant for understanding the pathological basis of these disorders. Researchers focus on Ndufs1 to unravel its impact on cellular metabolism and potential therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844068954281,"sku":"ab169540","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab169540","provider":"Iright","version":"1.0","type":"link"}