{"product_id":"abcam-ab176640","title":"Abcam, ab176640, ERK1\/2 (pT202\/Y204) ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nERK1\/2 (pT202\/Y204) ELISA Kit is a single-wash 90-min Simplestep used to quantify ERK1\/2 (pT202\/Y204) with a sensitivity of 0.1 ng\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Cited in over 10 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Homogenate, Cell Lysate,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nSensitivity:= 0.1 ng\/mL,\u003cbr\u003e\nRange:0.2 - 20 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nERK1\/2 (pT202\/Y204) ELISA Kit ab176640 is a rapid single-wash 90-min Other Immunoassay to measure ERK1\/2 (pT202\/Y204) in cell lysate, tissue homogenate. This SimpleStep sensitivity is 0.1 ng\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nERK1\/2 (pT202\/Y204) SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nERK1\/2 (pT202\/Y204) ELISA Kit ab176640 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nERK1 and ERK2 also known as MAPK3 and MAPK1 respectively are protein kinases involved in the mitogen-activated protein kinase (MAPK) signaling pathway. They share high sequence identity and exhibit similar functions. ERK1 has a molecular weight of approximately 44 kDa while ERK2 weighs around 42 kDa. Both are expressed ubiquitously in various tissues playing roles in diverse cellular processes. These proteins often detected through ERK1\/2 western blot analyses present similar ERK protein size and ERK molecular weight characteristics relevant during protein expression studies.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nERK1 and ERK2 play key roles in cell proliferation differentiation and survival. They form part of a cascade that includes upstream activators such as MEK1\/2 and downstream targets including transcription factors. As components of the MAPK signaling complex ERK1\/2 regulate gene expression through phosphorylation events impacting cellular responses to various stimuli. Their activation often hinges on growth factors cytokines and stress signals facilitating cellular adaptation to environmental changes.\u003cbr\u003e\nPathways\u003cbr\u003e\nRegarding pathways ERK1\/2 sit within the MAPK\/ERK pathway and are significant in the Ras\/Raf\/MEK\/ERK cascade one of the foremost signaling mechanisms in cells. They interact with several proteins including Ras and Raf which modulate their activation. This pathway is important for transmitting signals from the cell surface to the DNA in the cell nucleus impacting gene regulation and cell fate decisions. ERK1\/2 proteins therefore serve as critical nodes linking extracellular signals to cellular responses ensuring balanced cell function.\u003cbr\u003e\nERK1\/2 play significant roles in cancer and neurodegenerative diseases. Their overactivation is often linked to oncogenic processes contributing to cell proliferation in cancers. Mutations or dysregulation within the MAPK\/ERK pathway including ERK1\/2 frequently associate with tumorigenesis. Additionally in neurodegenerative disorders like Alzheimer's disease altered ERK1\/2 activity may impact neuronal survival and function often through interaction with amyloid-beta and tau proteins further illustrating their importance in disease pathophysiology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843547025577,"sku":"ab176640","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab176640","provider":"Iright","version":"1.0","type":"link"}