{"product_id":"abcam-ab176956","title":"Abcam, ab176956, Recombinant human DNA Polymerase Kappa\/POLK protein","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nRecombinant human DNA Polymerase Kappa\/POLK protein is a Human Fragment protein, in the 1 to 560 aa range, expressed in Escherichia coli, with \u0026gt;90%, suitable for SDS-PAGE, ELISA, WB, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, ELISA, FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Active,\u003cbr\u003e\nAccession:Q9UBT6,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 6 - 8.5Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.14% Sodium phosphate\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDNA polymerase kappa also known as POLK or kappa polymerase is a specialized enzyme involved in DNA replication and repair. This protein weighs approximately 93 kDa. POLK is expressed across various human tissues with notable activity in testis and placenta. Mechanically DNA polymerase kappa participates in translesion synthesis a process that permits DNA replication to proceed past damaged or abnormal bases allowing cells to overcome blockages that conventional DNA polymerases cannot bypass.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDNA polymerase kappa plays a significant role in error-prone repair mechanisms contributing to DNA damage tolerance. It is not known to associate directly with a larger complex but works in concert with other translesion synthesis polymerases such as DNA polymerase eta. Through its action POLK introduces nucleotides opposite DNA lesions maintaining replication fork progression and cell viability under genotoxic stress conditions.\u003cbr\u003e\nPathways\u003cbr\u003e\nDNA polymerase kappa is integral to the translesion synthesis pathway and participates in the broader DNA damage response system. It relates functionally to other DNA polymerases including polymerase eta and zeta which together modulate cellular responses to DNA damage and repair. The interplay between these enzymes facilitates flexible and adaptive responses to various genotoxic insults highlighting their roles in maintaining genomic stability.\u003cbr\u003e\nDNA polymerase kappa's association with carcinogenesis is of particular interest. Its error-prone activity can result in mutagenesis linking it to cancer development. POLK's expression or malfunction influences conditions like xeroderma pigmentosum where defective DNA repair increases cancer risk. Connections with the p53 pathway illustrate that defects in related DNA repair proteins can exacerbate the development of neoplastic diseases highlighting considerable implications for targeted cancer therapies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843842855081,"sku":"ab176956","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab176956","provider":"Iright","version":"1.0","type":"link"}