{"product_id":"abcam-ab177157","title":"Abcam, ab177157, Anti-AHAS antibody [EPR12843]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal AHAS antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR12843,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe acetohydroxyacid synthase (AHAS) also known as acetolactate synthase (ALS) is an enzyme that plays an important role in the biosynthesis of branched-chain amino acids. With a molecular mass of approximately 170 kDa AHAS catalyzes the first common step in the biosynthetic pathways of valine leucine and isoleucine. This enzyme is widely expressed in plants bacteria and fungi. AHAS is a holoenzyme that consists of a large catalytic subunit and a smaller regulatory subunit showing its complex structure and diverse regulatory control.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAHAS facilitates the condensation of two pyruvate molecules to form acetolactate or a pyruvate and ketoisovalerate to form acetohydroxybutyrate. This condensation reaction is a critical step in the synthesis of all three branched-chain amino acids which are essential components for protein construction in various organisms. As a part of a multi-enzyme complex the activity of AHAS is also modulated by feedback mechanisms from the end products of its pathway ensuring the balance in amino acid production and cellular homeostasis.\u003cbr\u003e\nPathways\u003cbr\u003e\nAHAS integrates into the branched-chain amino acid biosynthesis pathway directly taking its place at the start. This pathway connects to amino acid metabolism which is essential for protein synthesis. AHAS is intertwined with proteins like dihydroxyacid dehydratase and branched-chain aminotransferase which continue the pathway downstream. These interactions highlight AHAS's significance in creating amino acids necessary for organismal growth and development.\u003cbr\u003e\nAHAS relates prominently to conditions involving imbalances in amino acid metabolism. Alterations in AHAS function can lead to metabolic disorders such as maple syrup urine disease although it primarily involves branched-chain alpha-keto acid dehydrogenase complex. In some cases herbicides target AHAS poisoning the mechanism in plants and disrupting their growth. Understanding the function of AHAS helps us to comprehend the architecture of metabolic diseases and assists in designing therapeutic approaches even though the direct connection with human metabolic disorders remains mainly indirect.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850439872681,"sku":"ab177157","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab177157","provider":"Iright","version":"1.0","type":"link"}