{"product_id":"abcam-ab177487","title":"Abcam, ab177487, Anti-NeuN antibody [EPR12763] - Neuronal Marker","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) is a rabbit monoclonal antibody detecting NeuN in  Western Blot, Flow Cytometry, IHC-P, IHC-Fr, ICC\/IF, mIHC . Suitable for  Cat, Common marmoset, Dog, Human, Mouse, Rat, Sheep, Zebrafish . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 860 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR12763,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Sheep, Goat, Cat, Dog, Human, Zebrafish, Common marmoset,\u003cbr\u003e\nApplications:WB, Flow Cyt (Intra), IHC-Fr, mIHC, ICC\/IF, IHC-P, IHC (PFA fixed)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC\/IF, IHC (PFA fixed), IHC-Fr, IHC-P, WB, mIHC in cat, common marmoset, dog, human, mouse, rat, sheep, zebrafish samples.\u003cbr\u003e\nAnti-NeuN antibody (anti-RBFOX3 antibody) [EPR12763] - Neuronal Marker (ab177487) has been cited over 868 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure NeuN antigen antibody (ab177487) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nAnti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) has 72 independent reviews from customers.\u003cbr\u003e\nAnti-NeuN antibody [EPR12763] - Neuronal Marker (ab177487) specifically detects NeuN (UniProt ID: A6NFN3; Molecular weight: 34kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone EPR12763 -\u003cbr\u003e\nab209898\u003cbr\u003e\nAntibody clone EPR12763 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Biotin, Alexa Fluor® 594 (\u003cbr\u003e\nab190195\u003cbr\u003e\nab190565\u003cbr\u003e\nab204681\u003cbr\u003e\nab207279\u003cbr\u003e\nNeuN (RBFOX3) is a protein extensively used as a neuronal marker due to its expression in the nuclei and cytoplasm of neurons. It is vital for the regulation of alternative splicing of pre-mRNA, which is essential for neural development and function. Mutations or dysregulation of RBFOX3 have been associated with various neurological conditions, including autism spectrum disorder, cognitive deficits, and epilepsy, highlighting its importance in maintaining normal brain function.\u003cbr\u003e\nBrowse our curated portfolio\u003cbr\u003e\nof extensively validated antibodies and assays, with antibodies to over 90% of key targets in major glioblastoma pathways– including EGF, VEGF, MAPK, Wnt, NFKB, and PI3K\/AkT\/MTOR – all in one place.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nShipping conditions update: ambient shipping\u003cbr\u003e\nThis product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.\u003cbr\u003e\nWhy the change?\u003cbr\u003e\nIt’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.\u003cbr\u003e\nWhat you need to know\u003cbr\u003e\nAmbient shipments come clearly marked on the delivery note.\u003cbr\u003e\nNo ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.\u003cbr\u003e\nWarranty coverage remains fully valid, aligned with our validated shipping method.\u003cbr\u003e\nPlease store the product as per the datasheet instructions upon receipt.\u003cbr\u003e\nFind out more -\u003cbr\u003e\nhttps:\/\/www.abcam.com\/en-us\/support\/shipping-storage-support\/ambient-shipping\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNeuN also known as Fox-3 is a protein often utilized as a neuronal marker due to its expression predominantly in neurons. The molecular weight of NeuN is approximately 46-48 kDa. It is located in the nucleus and in the post-mitotic neurons of the central nervous system. Research frequently uses anti-NeuN antibodies for identifying neuronal cells in various samples capitalizing on its reliable expression profile across diverse neuron types. Common techniques for detecting NeuN include Western blot and immunohistochemistry (IHC) with NeuN staining being a definitive method for confirming neuronal identity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNeuN plays a role in RNA splicing specific to neurons contributing to the regulation of gene expression within the nervous system. It does not function as part of a larger protein complex but interacts with chromatin to influence neuronal gene expression. This has implications for neuronal differentiation and maintenance. Techniques like NeuN IHC use antibodies such as Alexa Fluor 568 conjugated variants to visualize neurons in tissue confirming its utility as an accessible and specific neuronal marker in research.\u003cbr\u003e\nPathways\u003cbr\u003e\nNeuN serves an important function in neuronal development and differentiation. It particularly influences neurogenesis and synaptogenesis pathways where neuron-specific splicing and transcriptional regulation are essential. Although NeuN does not directly engage in pathways with other proteins because it operates independently its expression parallels the activity of significant neuronal proteins such as MAP2 and Neurofilaments. These relationships illustrate NeuN's indirect association in pathways promoting neuronal development and maintenance.\u003cbr\u003e\nNeuN remains significant in studies of neurodegenerative diseases and neuronal damage. For instance decreased NeuN staining has been observed in Alzheimer's disease indicating loss of neuronal identity or survival. Research also notes alterations in NeuN expression following ischemic injury where its presence or absence links to neuronal viability. NeuN's association with these states of neuronal health or pathology highlights its relevance as a marker in neurodegenerative and injury-related disorders. Furthermore while not directly in disease pathways NeuN functionally intersects with proteins like Tau in Alzheimer's suggesting its indirect role in disease pathways.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844025110697,"sku":"ab177487","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab177487","provider":"Iright","version":"1.0","type":"link"}