{"product_id":"abcam-ab181104","title":"Abcam, ab181104, Anti-RNF20 antibody [EPR13562(B)]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal RNF20 antibody. Suitable for ICC\/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR13562(B),\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IP, Flow Cyt (Intra), ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRNF20 also known as BRE1A or E3 ubiquitin-protein ligase RNF20 mechanically functions as an E3 ligase that mediates ubiquitination. Its mass is approximately 110 kDa. RNF20 is expressed across various tissues with a high presence in the spleen and testis. It specifically adds ubiquitin to lysine residues on target histones leading to changes in chromatin structure and gene expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRNF20 works within the ubiquitination system and forms part of the RNF20\/RNF40 complex. This complex plays a role in the monoubiquitination of histone H2B at lysine 120 (H2BK120ub) a significant post-translational modification influencing transcriptional regulation. The modification aids in recruiting downstream effectors and is important for gene expression regulation and DNA damage repair mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nRNF20 interacts with several cellular processes including the DNA damage response and transcriptional activation pathways. It significantly contributes to the DNA repair pathway by facilitating recruitment of repair proteins and is also involved in transcription regulation through the histone modification pathway. Furthermore RNF20 shows a functional relationship with RNF40 another E3 ligase in these pathways emphasizing its integrative role in cellular functioning.\u003cbr\u003e\nDisruptions in RNF20 activity have connections to cancer and neurodegenerative disorders. Altered RNF20 expression or mutations can influence tumor growth due to impaired regulation of gene expression and DNA repair impacting proteins like p53. Additionally in neurodegenerative disorders anomalies in RNF20-mediated ubiquitination can affect protein homeostasis linking to proteins involved in neuronal integrity and survival.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850428993705,"sku":"ab181104","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab181104","provider":"Iright","version":"1.0","type":"link"}