{"product_id":"abcam-ab181557","title":"Abcam, ab181557, Anti-Oct4 antibody [EPR17929] - ChIP Grade","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) is a rabbit monoclonal antibody detecting Oct4 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF, ChIP . Suitable for  Human, Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 130 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR17929,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:ChIP-seq, Flow Cyt (Intra), WB, ChIP, ICC\/IF, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Oct4 is highly expressed in undifferentiated embryonic stem cells and cancer stem cell-like cells (PMID: 26013162, 21826175).This antibody can't detect the target band in undifferentiated cancer cell lines with low expression level of Oct4, such as HeLa, HEK-293, MDA-MB-231, HepG2, Huh7, HCT-116 and PANC-1 (PMID: 21975933, 29789579, 25625591, 26059097, 23928699, 27344963, 25837691, 29254202, 28854261, 27996162), even at the dilution of 1:200.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIP, ChIP-seq, Flow Cyt (Intra), ICC\/IF, IHC-P, IP, WB in human, mouse samples.\u003cbr\u003e\nAnti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) specifically detects Oct4 (UniProt ID: Q01860; Molecular weight: 39kDa) and is sold in 100 µL and 1 mL selling sizes.\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nAnti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) has been cited over 138 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-Oct4 antibody [EPR17929] - ChIP Grade (ab181557) has 19 independent reviews from customers.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nAntibody clone EPR17929 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 594, Alexa Fluor® 555, PE, Alexa Fluor® 488, Alexa Fluor® 647 (\u003cbr\u003e\nab210458\u003cbr\u003e\nab210657\u003cbr\u003e\nab283731\u003cbr\u003e\nab283741\u003cbr\u003e\nab300092\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nOct4 also known as POU5F1 Oct 4 protein and hecho en Oct 4 is a transcription factor important for maintaining pluripotency in stem cells. Oct4 protein has a molecular weight of approximately 40-45 kDa. Stem and germ cells express Oct4 where it regulates gene expression essential for cell differentiation and self-renewal. In immunohistochemistry (IHC) studies scientists often use Oct4 staining to identify stem cell presence and behavior.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nOct4 acts in concert with other pluripotency factors like SOX2 and NANOG to form a regulatory network essential for maintaining stem cell identity. As part of this network the Oct4 transcription factor orchestrates the expression of genes that prevent differentiation and maintain the stem cell’s undifferentiated state. The protein directly activates the transcription of target genes involved in maintaining the pluripotent state.\u003cbr\u003e\nPathways\u003cbr\u003e\nOct4 plays an important role in the maintenance of pluripotency and self-renewal in the embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) pathways. Oct4 partners with proteins such as SOX2 and KLF4 in these pathways to activate and repress a common set of downstream genes which control differentiation and proliferation. Oct4 ensures the blocking of differentiation signals while promoting self-renewal in pluripotent cells.\u003cbr\u003e\nAberrant Oct4 expression often links to oncogenesis and tumor progression particularly in testicular germ cell tumors and certain types of cancers. Oct4 overexpression may interact with c-MYC a protein linked to cancer cell proliferation advancing tumorigenesis. Additionally researchers investigate Oct4 as a diagnostic marker for cancer and its potential as a therapeutic target to inhibit cancer cell growth.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844032581801,"sku":"ab181557","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab181557","provider":"Iright","version":"1.0","type":"link"}