{"product_id":"abcam-ab18180","title":"Abcam, ab18180, Anti-ABCA1 antibody [AB.H10]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-ABCA1 antibody [AB.H10] (ab18180) is a mouse monoclonal antibody detecting ABCA1 in  Western Blot, Flow Cytometry, IHC-P . Suitable for  Human, Mouse . - KO validated for confirmed specificity - Over 200 publications - Trusted since 2005\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:AB.H10,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:Flow Cyt, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human ABCA1 aa 1800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O95477\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-ABCA1 antibody [AB.H10] (ab18180) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse samples.\u003cbr\u003e\nWhat is the molecular weight of ABCA1?\u003cbr\u003e\nAnti-ABCA1 [AB.H10] (ab18180) specifically detects a band for ABCA1 (UniProt: O95477) at a molecular weight of 254kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-ABCA1 [AB.H10] (ab18180) was first used in a scientific publication in 2005 and has been cited over 200 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-ABCA1 [AB.H10] (ab18180) has over 15 independent reviews from customers.\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-ABCA1 antibody [AB.H10] (ab18180) has been confirmed by Western blot testing in ABCA1 Knockout Mouse liver samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Protein A purified from TCS, Storage buffer-pH: 7.2Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ABCA1 protein also known as ATP-binding cassette transporter A1 plays an important role in lipid transport processes. It helps in the export of cholesterol and phospholipids to apolipoprotein A-I which is essential for high-density lipoprotein (HDL) formation. The ABCA1 molecular weight is approximately 220 kDa. This protein is widely expressed in various tissues including the liver lungs and intestines indicating its systemic role in lipid metabolism. Researchers often study ABCA1 using techniques like ABCA1 ELISA and H10 flow cytometry.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nABCA1 functions as a cholesterol efflux pump in the cellular membrane. It facilitates the transfer of cholesterol to lipid-poor apolipoproteins such as apoA-I which then forms HDL particles. This active process helps maintain cellular cholesterol homeostasis and reduces cholesterol accumulation. ABCA1 is a critical component of the HDL synthesis pathway and does not form part of a larger protein complex acting more as an individual unit in this function.\u003cbr\u003e\nPathways\u003cbr\u003e\nMany regulatory mechanisms control the activity of ABCA1. It plays a significant role in the reverse cholesterol transport pathway thereby promoting the movement of cholesterol away from peripheral tissues back to the liver for excretion. This pathway helps maintain systemic lipid balance. The LXR\/RXR pathway regulates ABCA1 expression aligning it closely with other proteins such as LXR and ABCG1 which also deal with cholesterol metabolism.\u003cbr\u003e\nABCA1 is intimately connected with atherosclerosis and Tangier disease. Dysfunction or mutations in the ABCA1 gene lead to impaired cholesterol efflux contributing to cholesterol build-up and atherosclerotic plaque formation. Tangier disease a rare genetic disorder arises from severe mutations in ABCA1 resulting in very low levels of HDL cholesterol. This protein's role in disease is often studied alongside proteins such as apoA-I and LDL receptors which influence cholesterol transportation and storage.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844227584169,"sku":"ab18180","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab18180","provider":"Iright","version":"1.0","type":"link"}