{"product_id":"abcam-ab18207","title":"Abcam, ab18207, Anti-beta III Tubulin antibody - Neuronal Marker","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-beta III Tubulin antibody - Neuronal Marker (ab18207) is a rabbit polyclonal antibody detecting beta III Tubulin in  Western Blot, Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Catshark, Common marmoset, Dogfish, Human, Mouse, Rat . - Over 620 publications - Trusted since 2005\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human, Common marmoset, Dogfish, Catshark,\u003cbr\u003e\nApplications:ICC\/IF, Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The immunogen used for this product shares 75% homology with TUB (Tubby protein homolog, Uniprot: P50607). In western blot, we observe a specific band at ~55kDa which is not seen in KO cell lines. An additional band below this band of interest is seen at ~50kDa in both the WT and KO cells which could correspond to the protein TUB. Please note that cross-reactivity with this protein has not been confirmed experimentally. TUB is localized notably in high concentrations in the nucleoli of brain neurons with lower protein levels in the cytoplasm. Please, therefore, be aware that ICC experiments may need to be optimised. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. As an alternative antibody, we would recommend our recombinant rabbit monoclonal antibody ab52623 which has been shown to specific in both WB and ICC using KO cells.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-beta III Tubulin antibody - Neuronal Marker (ab18207) is a rabbit polyclonal antibody and is validated for use in Flow Cyt (Intra), ICC\/IF, IHC-P, WB.\u003cbr\u003e\nAnti-beta III Tubulin antibody - Neuronal Marker (ab18207) has been cited over 627 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) has high sensitivity and specificity.\u003cbr\u003e\nThe specificity of Anti-beta III Tubulin antibody - Neuronal Marker (ab18207) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-beta III Tubulin antibody - Neuronal Marker (ab18207) has 67 independent reviews from customers.\u003cbr\u003e\nbeta III Tubulin antibodies are often used as loading controls in Western Blot. Anti-beta III Tubulin antibody - Neuronal Marker has been verified in Western Blot samples and detects a band at 50kDa Molecular weight.\u003cbr\u003e\nAnti-beta III Tubulin antibody - Neuronal Marker (ab18207) specifically detects beta III Tubulin (UniProt ID: Q13509; Molecular weight: 50kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nBeta-tubulin III (TUBB3) is a protein that is predominantly expressed in neurons and is a critical component of the microtubule network. It plays a vital role in maintaining the structure and function of neurons. It is a key marker for neurons and is involved in processes such as axon guidance and neuronal differentiation. Abnormal expression of TUBB3 has been associated with several neurological conditions, including congenital fibrosis of the extraocular muscles (CFEOM) and cortical dysplasia, complex with other brain malformations (CDCBM), underscoring its critical role in brain development.\u003cbr\u003e\nBrowse our curated portfolio\u003cbr\u003e\nof extensively validated antibodies and assays, with antibodies to over 90% of key targets in major glioblastoma pathways– including EGF, VEGF, MAPK, Wnt, NFKB, and PI3K\/AkT\/MTOR – all in one place.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nBeta III Tubulin also known as beta3 tubulin beta tubulin III and bIII tubulin is a microtubule component that plays integral mechanical roles in cells. With a mass of approximately 55 kDa this protein is part of the tubulin family. You find beta III tubulin predominantly in neurons within the central and peripheral nervous systems. However it sometimes appears in other tissues under certain conditions like stress or disease.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nBeta III tubulin contributes to neuronal differentiation and axonal guidance due to its incorporation into the microtubule network. As a component of the microtubule complex beta III tubulin regulates the dynamic instability of microtubules influencing their assembly and disassembly rates. Its expression in developing neurons supports growth cones as they extend axons to their targets facilitating proper neuronal circuit formation.\u003cbr\u003e\nPathways\u003cbr\u003e\nBeta III tubulin actively participates in the neuronal microtubule dynamics pathway. This pathway is essential for the development and maintenance of neuronal structure and function. Beta III tubulin interacts closely with other tubulin isoforms and microtubule-associated proteins (MAPs) to ensure proper microtubule function. In addition the protein indirectly influences the MAPK signaling pathway which affects cellular processes such as growth and differentiation.\u003cbr\u003e\nBeta III tubulin has strong links to certain neurological conditions and cancers. In neurodegenerative disorders like Alzheimer's disease abnormal regulation of beta III tubulin contributes to impaired neuronal functions. Additionally it is associated with cancers like glioblastoma where upregulated expression is used as a marker for diagnosis and prognosis. Through its involvement in these diseases beta III tubulin indirectly affects interactions with proteins involved in cellular stress responses and neurodegeneration such as tau protein.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844065874089,"sku":"ab18207","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab18207","provider":"Iright","version":"1.0","type":"link"}