{"product_id":"abcam-ab185906","title":"Abcam, ab185906, Post-Bisulfite DNA Library Preparation Kit (For Illumina®)","description":"\u003cp\u003eSize: 12Test \/ 24Test\u003cbr\u003e\nab185906 is a complete set of optimized reagents to prepare a DNA library - after successful bisulfite conversion - for various Illumina® platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representative bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nAssay time:5h,\u003cbr\u003e\nRange:0.5 - 1000 ng,\u003cbr\u003e\nSensitivity:= 1 ng\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab185906 is a complete set of optimized reagents to prepare a DNA library - after successful bisulfite conversion - for various Illumina® platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representative bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications. The optimized protocol and components of the kit allow both non-barcoded (singleplexed) and barcoded (multiplexed) DNA libraries to be quickly constructed using sub-nanogram input concentrations of DNA since the DNA is first bisulfite-converted and then used for library preparation.\u003cbr\u003e\nIllumina® is a registered trademark of Illumina, Inc.\u003cbr\u003e\nSeveral methods, including whole genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS), are currently used for genome-wide DNA methlyation analysis. These methods convert unmethylated cytosines to uracil while 5-methylcytosines remain unchanged by the bisulfite treatment. This allows epigenetic differences to become genetic differences, which can be subsequently detected via sequencing at the single-based resolution level and on a genome-wide scale. However, practical use with such current methods are not ideal as they (1) need large amounts of DNA (\u0026gt;1 μg) as input material, which is difficult to prepare from limited biological samples such as tumor biopsy, early embyros, embyronic tissues, and circulating DNA; (2) require DNA to first be sheared and then ligated to adapters, followed by bisulfite conversion (post-ligation bisulfite conversion), which causes substantial amounts of DNA fragments contained in the adapter-DNA fragment constructs to be broken and thereby forms mono-tagged templates that become removed during library enrichment; and (3) are time-consuming (2 days). The Post-Bisulfite DNA Library Preparation Kit (For Illumina®) is designed to overcome these weaknesses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847699484841,"sku":"ab185906","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab185906","provider":"Iright","version":"1.0","type":"link"}