{"product_id":"abcam-ab185907","title":"Abcam, ab185907, Bisulfite-Seq High Sensitivity Kit (For Illumina®)","description":"\u003cp\u003eSize: 12Test \/ 24Test\u003cbr\u003e\nab185907 is designed to carry out bisulfite conversion, followed by a \"post-bisulfite\" library preparation process for Illumina® platform-based bisulfite sequencing, all in one kit.\u003cbr\u003e\nKey facts\u003cbr\u003e\nAssay time:8h,\u003cbr\u003e\nSensitivity:\u0026lt; 0.5 ng\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab185907 is designed to carry out bisulfite conversion, followed by a \"post-bisulfite\" library preparation process for Illumina® platform-based bisulfite sequencing, all in one kit. The DNA library is constructed directly from bisulfite-converted DNA generated from a small amount of input DNA (500 pg to 500 ng). Intended applications include whole genome bisulfite sequencing, oxidative bisulfite sequencing, reduced representative bisulfite sequencing, and various other bisulfite-next generation sequencing techniques. The optimized protocol and components of the kit allow the DNA to be bisulfite converted and fragmented simultaneously followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using sub-nanogram quantities of bisulfite converted DNA.\u003cbr\u003e\nDNA methylation occurs by the covalent addition of a methyl group (CH\u003cbr\u003e\n) at the 5-carbon of the cytosine ring, resulting in 5-methylcytosine (5-mC). DNA methylation is essential in regulating gene expression in nearly all biological processes including development, growth, and differentiation. Alterations in DNA methylation have been demonstrated to cause a change in gene expression. Genome-wide analysis of DNA methylation could provide valuable information for discovering epigenetic markers used for disease diagnosis, and potential targets used for therapeutics. Bisulfite sequencing via next-generation sequencing technologies allow for high volume, lower cost output of DNA sequence data towards a better understanding of DNA methylation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847699615913,"sku":"ab185907","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab185907","provider":"Iright","version":"1.0","type":"link"}