{"product_id":"abcam-ab185986","title":"Abcam, ab185986, Human IL-10 ELISA Kit (Interleukin-10)","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests\u003cbr\u003e\nHuman IL-10 ELISA kit (Interleukin-10) is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human IL-10 with a sensitivity of 1.4 pg\/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450 nm readout; works on any plate reader - Different formats for different needs: 384-well for higher throughput - Broad sample compatibility – works with serum, plasma, and cell culture supernatants - Cited in over 30 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.4 pg\/mL,\u003cbr\u003e\nRange:9.4 - 3000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-10 ELISA Kit (Interleukin-10) ab185986 is a rapid single-wash 90-min ELISA kit to measure Human IL-10 in cell culture supernatant, serum, plasma. This SimpleStep ELISA® sensitivity is 1.4 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman IL-10 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit is designed for the quantification of human IL-10. The standard protein in this kit is mature full length human IL-10. Native signal was detected in cell culture supernatant. Spike protein experiments were used to validate serum and plasma samples. Saliva, milk, CSF, cell extract and tissue extract samples have not bee tested with this kit. 50% pooled serum and plasma samples from healthy donors were measured in duplicate. All values were below the detectable range of this assay. 50% serum from 10 healthy human male donors was measured in duplicate. All values were below the detectable range of this assay.\u003cbr\u003e\nHuman IL-10 ELISA Kit (Interleukin-10) ab185986 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Human IL-10 ELISA Kit ab185986\u003cbr\u003e\nHuman IL-10 ELISA Kit ab185986 has been used to assess inflammation in different physio-pathological contexts such as celiac disease\u003cbr\u003e\n, tuberculosis\u003cbr\u003e\n, and atherosclerosis\u003cbr\u003e\nReferences:\u003cbr\u003e\nSoroush Sardari et al. 2023, PMID: 38040898,\u003cbr\u003e\nHao Zeng et al. 2023, PMID: 37601387,\u003cbr\u003e\nYoujiang Qiu et al. 2023, PMID: 36820569.\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human IL-10 ELISA Kit (Interleukin-10) only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human IL-10 Antibody Pair - BSA and Azide free (Interleukin-10)\u003cbr\u003e\nab243976\u003cbr\u003e\n- Anti-IL-10 antibody [EPR20433-201] - BSA and Azide free (Capture)\u003cbr\u003e\nab244835\u003cbr\u003e\n- Anti-IL-10 antibody [EPR20433-290] - BSA and Azide free (Detector)\u003cbr\u003e\nab244889\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman IL-10 ELISA Kit ab185986 is commonly used to measure IL-10 as a marker of inflammation.\u003cbr\u003e\nOther ELISA kits to measure inflammatory markers include:\u003cbr\u003e\n- Human TNF alpha ELISA kit\u003cbr\u003e\nab181421\u003cbr\u003e\n- Human IL-8 ELISA kit\u003cbr\u003e\nab214030\u003cbr\u003e\n- Human IL-6 ELISA kit\u003cbr\u003e\nab178013\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843537260713,"sku":"ab185986","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab185986","provider":"Iright","version":"1.0","type":"link"}