{"product_id":"abcam-ab193258","title":"Abcam, ab193258, Protein G Agarose (High Affinity)","description":"\u003cp\u003eSize: 5mL \/ 25mL \/ 100mL\u003cbr\u003e\nProtein G Agarose (High Affinity) (ab193258) contains recombinant Protein G coupled to highly cross-linked 4% agarose beads. Suitable for antibody purification and immunoprecipitation (IP) assays. - Binding Capacity: \u0026gt;30 mg Goat lgG\/mL medium - Used for monoclonal and polyclonal antibody purification - Available in different test sizes to fit your experimental needs\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:Purification, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Constituents: 20% Ethanol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nVery high binding capacity (\u0026gt;30 mg IgG\/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG, immunoprecipitation \u0026amp; ChIP (ab193258).\u003cbr\u003e\nContents:\u003cbr\u003e\nSupplied as 50% slurry in 20% Ethanol.\u003cbr\u003e\nFeatures:\u003cbr\u003e\nHigh binding capacity: Binding of IgG ≥ 30 mg human or rabbit IgG\/mL Protein G Agarose.\u003cbr\u003e\nMinimal leaching of the ligand\u003cbr\u003e\nFlow Rate Tested*: 2.89 mL\/min.\u003cbr\u003e\n*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).\u003cbr\u003e\nMaximum Flow Rate**: 1800 cm\/hr; minimum leaching of recombinant Protein G.\u003cbr\u003e\n**NOTE: the highest flow that beads withstand for 1 minute, without collapsing and the pressure reaching 1 MPa.\u003cbr\u003e\nUsage:Reusable for up to 10 times without significant loss of binding capacity.\u003cbr\u003e\nThese beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x\u003cbr\u003e\nwhen centrifuging.\u003cbr\u003e\nStore beads at 4°C.\u003cbr\u003e\nThe beads may be damaged above 40°C.\u003cbr\u003e\nDO NOT FREEZE.\u003cbr\u003e\nWash beads 3 times with 3x bead volume of desired buffer before use.\u003cbr\u003e\nApplications:\u003cbr\u003e\n- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.\u003cbr\u003e\n- Isolation of antibody\/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called 6513 Hi-Bind™ Protein G-Agarose. 6513-5 is the same size as the 5 ml size of ab193258.\u003cbr\u003e\nProtein G Agarose (High Affinity) beads are specially prepared for high IgG binding by covalently coupling recombinant Protein G to 6% cross-linked agarose beads, the most popular resin for protein affinity purification methods. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein G than standard Protein G agarose beads. The IgG binding capacity of Protein G Agarose (High Affinity) is ≥ 30 mg of human or rabbit IgG per mL of wet beads. The Protein G Agarose (High Affinity) beads display high chemical and physical stability, as well as high flow rate, hydrophilicity and high gel strength.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847574802601,"sku":"ab193258","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab193258","provider":"Iright","version":"1.0","type":"link"}