{"product_id":"abcam-ab194124","title":"Abcam, ab194124, Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-Cytokeratin 18 antibody [E431-1] - Alexa Fluor® 488 conjugated (ab194124) is a rabbit recombinant monoclonal antibody detecting Cytokeratin 18 in  Flow Cytometry (Intra), Flow Cytometry, ICC\/IF . Suitable for  Human . - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:E431-1,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nConjugation:Alexa Fluor® 488,\u003cbr\u003e\nExcitation\/Emission:Ex: 495nm, Em: 519nm,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:Flow Cyt (Intra), ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAlexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1] (ab194124) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nRelated products\u003cbr\u003e\nAntibody clone E431-1 is also available pre-conjugated to a variety of labels for your convenience – Anti-Cytokeratin 18 Alexa Fluor® 488 [E431-1] (ab194124).\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [E431-1] also available for your convenience:\u003cbr\u003e\nab32118\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab193557\u003cbr\u003e\n, Alexa Fluor® 488 - ab194124, HRP -\u003cbr\u003e\nab194126\u003cbr\u003e\n, PE -\u003cbr\u003e\nab210410\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab283629\u003cbr\u003e\n, Carrier free - ab302463\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nAlexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle|Stable for 12 months at -20°C|Store in the dark\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.\u003cbr\u003e\nPathways\u003cbr\u003e\nCytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.\u003cbr\u003e\nElevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848563380393,"sku":"ab194124","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab194124","provider":"Iright","version":"1.0","type":"link"}