{"product_id":"abcam-ab194518","title":"Abcam, ab194518, Anti-STAT5a (phospho S725) + STAT5b (phospho S730) antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal STAT5a phospho S725 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human STAT5A phospho S725 conjugated to Keyhole Limpet Haemocyanin.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.Synthetic Peptide within Human STAT5B phospho S730 conjugated to Keyhole Limpet Haemocyanin. Database linkP51692,\u003cbr\u003e\nSpecificity:ab194518 detects endogenous levels of STAT5a + STAT5b only when phosphorylated at serine 725 \/ serine 730.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab194518 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSTAT5a and STAT5b known jointly as members of the Signal Transducers and Activators of Transcription family play critical roles as transcription factors. They have molecular masses of approximately 94 kDa and 92 kDa respectively. These proteins share significant sequence similarity and are expressed in a wide range of tissues including hematopoietic cells liver and mammary glands. STAT5 proteins become activated in response to cytokines and growth factors where they dimerize and translocate to the cell nucleus to regulate gene expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThey are involved in mediating cytokine signaling by forming homo- or heterodimers upon phosphorylation by Janus kinases (JAKs). In the cytoplasm the formation of these dimers allows STAT5 to interact with DNA in the nucleus leading to the transcription of various genes important for cell proliferation differentiation and apoptosis. STAT5 has a significant role in cell cycle control and immune responses. The dimerization process involves complex formation and is an important part of its intracellular signaling cascade.\u003cbr\u003e\nPathways\u003cbr\u003e\nSTAT5a and STAT5b operate prominently within the JAK-STAT pathway. They are activated by proteins such as IL-2 IL-3 and Growth Hormone leading to transcriptional activity that affects growth and survival across various cell types. Besides the JAK-STAT pathway STAT5 interacts closely with the PI3K\/AKT pathway thereby linking to cell survival signals. The cross-talk between these pathways enables fine-tuning of cellular responses to external stimuli and integrates signals from diverse receptors and ligands.\u003cbr\u003e\nSTAT5 deregulated activity associates with various cancers such as leukemia and breast cancer due to its role in promoting excessive cell proliferation and survival. Abnormal activity in STAT5 can also contribute to disorders like autoimmune diseases due to its impact on immune cell regulation. In diseases like these STAT5 interacts notably with proteins such as BCL-2 and MYC which are critical modulators of cell death and growth pathways. Understanding the involvement of STAT5 in disease mechanisms provides opportunities for developing targeted therapeutics.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848633536681,"sku":"ab194518","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab194518","provider":"Iright","version":"1.0","type":"link"}