{"product_id":"abcam-ab194534","title":"Abcam, ab194534, Anti-CAD (phospho T456) antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal CAD phospho T456 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CAD protein phospho T456 conjugated to Keyhole Limpet Haemocyanin.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human CAD protein phospho T456 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P27708\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab194534 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe CAD protein also known as carbamoyl-phosphate synthase 2\/ aspartate transcarbamylase\/ dihydroorotase serves an important mechanical role in pyrimidine biosynthesis. CAD is a multi-functional enzyme with a molecular mass of approximately 243 kDa and is expressed in the cytoplasm of many cell types. This complex protein organization enables it to catalyze the first three steps of de novo pyrimidine synthesis involving glutamine-dependent carbamoyl phosphate synthesis aspartate transcarbamylation and dihydroorotase activity. Such vital tasks establish CAD as an important player in the nucleotide biosynthesis landscape.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe CAD enzyme facilitates the production of pyrimidine nucleotides that are necessary for DNA and RNA synthesis. CAD operates as a single-polypeptide chain trifunctional enzyme and is part of a larger protein complex involved in nucleic acid metabolism. Its enzymatic activities align closely to support cellular proliferation and differentiation processes that demand efficient nucleotide production. This link between CAD function and nucleotide availability underlines its importance in ensuring smooth cell cycle progression and maintenance.\u003cbr\u003e\nPathways\u003cbr\u003e\nCAD integrates into the broader nucleotide metabolism and pyrimidine biosynthesis pathways. The CAD protein directly interacts with other key enzymes such as UMP synthase which furthers the conversion of its product dihydroorotate to UMP. OMP decarboxylase also acts downstream reflecting CAD's role in ensuring continuity and efficiency in pyrimidine synthesis. These interactions demonstrate CAD's central involvement in maintaining the nucleotide pool required for DNA synthesis and repair.\u003cbr\u003e\nCAD mutations and dysregulations link to neurological disorders and cancer. Neurological conditions such as CAD deficiency can result from impaired pyrimidine synthesis affecting brain development and function. Additionally enhanced CAD activity associates with certain cancers where its upregulated pyrimidine production supports rapid tumor cell proliferation. In these disease contexts CAD's interaction with proteins involved in cellular proliferation pathways such as c-Myc in cancers highlights its impact on health and disease states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848632619177,"sku":"ab194534","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab194534","provider":"Iright","version":"1.0","type":"link"}