{"product_id":"abcam-ab195224","title":"Abcam, ab195224, AMCA Conjugation Kit (Fast) - Lightning-Link®","description":"\u003cp\u003eSize: 100µg \/ 3 x 10µg \/ 3 x 100µg \/ 1mg\u003cbr\u003e\nAMCA Conjugation Kit (Fast) - Lightning-Link® (ab195224) offers several standout features: - Rapid Conjugation: achieve AMCA labeling in under 20 minutes with just 30 seconds of hands-on time. - High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies. - Versatility: suitable for conjugating antibodies, proteins, and peptides. AMCA-labeled antibodies can be used immediately in applications such as Western blot, ELISA, and Immunohistochemistry (IHC) without further purification.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nAMCA Conjugation Kit \/ AMCA Labeling Kit (ab195224) uses a simple and quick process for AMCA labeling \/ conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our\u003cbr\u003e\nantibody labeling kits and their advantages\u003cbr\u003e\nTo conjugate an antibody to AMCA using this kit:\u003cbr\u003e\n- add modifier to antibody and incubate for 15 mins\u003cbr\u003e\n- add quencher and incubate for 5 mins\u003cbr\u003e\nThe AMCA conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.\u003cbr\u003e\nLearn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid\u003cbr\u003e\nantibody purification and concentration kits\u003cbr\u003e\n. Use the\u003cbr\u003e\nto learn more about the technology, or about conjugating other proteins and peptides to AMCA.\u003cbr\u003e\nCustom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link\u003cbr\u003e\nRapid AMCA Labeling Kit. 313-0015 is the same as the 1 mg size. 313-0030 is the same as the 3 x 10 ug size. 313-0010 is the same as the 3 x 100 ug size. 313-0005 is the same as the 100 ug size.\u003cbr\u003e\nAmount and volume of antibody for conjugation to AMCA\u003cbr\u003e\nKit size\u003cbr\u003e\nRecommended\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum antibody\u003cbr\u003e\nvolume\u003cbr\u003e\n3 x 10 μg\u003cbr\u003e\n3 x 10 μg\u003cbr\u003e\n3 x 20 μg\u003cbr\u003e\n3 x 10 μL\u003cbr\u003e\n100 μg\u003cbr\u003e\n1 x 100 μg\u003cbr\u003e\n1 x 200 μg\u003cbr\u003e\n1 x 100 μL\u003cbr\u003e\n3 x 100 μg\u003cbr\u003e\n3 x 100 μg\u003cbr\u003e\n3 x 200 μg\u003cbr\u003e\n3 x 100 μL\u003cbr\u003e\n1 x 1 mg\u003cbr\u003e\n1 x 2 mg\u003cbr\u003e\n1 x 1 mL\u003cbr\u003e\nUsing the maximum amount of antibody may result in less labelling per antibody.\u003cbr\u003e\nIdeal antibody concentration is 1mg\/ml. 0.5 - 1 mg\/ml can be used if the maximum antibody volume is not exceeded. Antibodies \u0026gt; 2mg\/ml or \u0026lt; 0.5 mg\/ml should be diluted \/concentrated.\u003cbr\u003e\nBuffer Requirements for Conjugation\u003cbr\u003e\nBuffer should be pH 6.5-8.5.\u003cbr\u003e\nCompatible buffer constituents\u003cbr\u003e\nIf a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.\u003cbr\u003e\n50mM \/ 0.6% Tris\u003cbr\u003e\n0.1% BSA\u003cbr\u003e\n50% glycerol\u003cbr\u003e\n0.1% sodium azide\u003cbr\u003e\nPotassium phosphate\u003cbr\u003e\nSodium chloride\u003cbr\u003e\nSucrose\u003cbr\u003e\nSodium citrate\u003cbr\u003e\nTrehalose\u003cbr\u003e\nTris buffered saline is almost always ≤ 50 mM \/ 0.6%\u003cbr\u003e\nBSA can also interfere with the use of the conjugated antibody in tissue staining.\u003cbr\u003e\nIncompatible buffer constituents\u003cbr\u003e\nThiomerosal\u003cbr\u003e\nProclin\u003cbr\u003e\nGlycine\u003cbr\u003e\nArginine\u003cbr\u003e\nGlutathione\u003cbr\u003e\nIf a constituent of the buffer containing your antibody or protein is not listed above, please contact us.\u003cbr\u003e\nOnly purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.\u003cbr\u003e\nStoring and handling conjugation kits\u003cbr\u003e\nLyophilized Lightning-Link\u003cbr\u003e\ncomponents are hygroscopic.\u003cbr\u003e\nKits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847602229417,"sku":"ab195224","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab195224","provider":"Iright","version":"1.0","type":"link"}