{"product_id":"abcam-ab195380","title":"Abcam, ab195380, Anti-WTAP antibody [EPR18744]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-WTAP antibody [EPR18744] (ab195380) is a rabbit monoclonal antibody detecting WTAP in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR18744,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IP, ICC\/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-WTAP antibody [EPR18744] (ab195380) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of WTAP?\u003cbr\u003e\nAnti-WTAP [EPR18744] (ab195380) specifically detects a band for WTAP (UniProt: Q15007) at a molecular weight of 44kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-WTAP [EPR18744] (ab195380) was first used in a scientific publication in 2016 and has been cited over 40 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-WTAP [EPR18744] (ab195380) has over 5 independent reviews from customers.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-WTAP antibody [EPR18744] (ab195380) has been confirmed by Western blot testing in WTAP Knockout HAP1 cells.\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR18744] also available for your convenience: ab195380, Alexa Fluor® 488 -\u003cbr\u003e\nab225404\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab232392\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nWTAP also known as Wilms tumor 1-associated protein is a core component of the writer complex that catalyzes N6-methyladenosine (m6A) methylation on mRNA. With a molecular mass of approximately 39 kDa WTAP localizes almost ubiquitously in human tissues but shows higher expression in liver and kidney cells. This protein assists in the regulation of mRNA splicing and stability functioning as an adapter for RNA binding proteins.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis protein plays a critical role in post-transcriptional gene regulation by being part of the m6A methyltransferase complex which includes other important proteins like METTL3 and METTL14. WTAP does not bind RNA directly but helps its partners achieve target specificity during gene expression processes. The presence of WTAP within the complex stabilizes and coordinates the activity of the entire methyltransferase assembly.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe m6A methylation WTAP facilitates affects various pathways including mRNA splicing and degradation. This process intricately connects WTAP to the regulation of the JAK-STAT signaling pathway where its activity influences inflammatory responses and cell proliferation. Additionally WTAP has ties to the Wnt\/Β-catenin pathway collaborating with proteins like METTL3 to regulate gene expression involved in cell fate determination and embryogenesis.\u003cbr\u003e\nResearch shows WTAP has connections to various cancers such as hepatocellular carcinoma due to its role in mRNA processing and stability. This association often involves its interaction with other oncogenic proteins like METTL3 leading to aberrant gene expression patterns. WTAP also has relevance in acute myeloid leukemia where it influences mRNA maturation and impacts cell differentiation and proliferation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844036219049,"sku":"ab195380","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab195380","provider":"Iright","version":"1.0","type":"link"}