{"product_id":"abcam-ab197022","title":"Abcam, ab197022, Anti-HMGCL antibody [EPR15551]","description":"\u003cp\u003eSize: 10µL-TRIAL \/ 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal HMGCL antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR15551,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHMGCL or 3-hydroxymethyl-3-methylglutaryl-CoA lyase plays an important role in ketogenesis and leucine catabolism. This enzyme catalyzes the cleavage of HMG-CoA to acetoacetate and acetyl-CoA. It has a molecular mass of approximately 33 kDa. HMGCL expresses mainly in the liver kidney and brain where ketogenesis is active signifying its essential role in energy metabolism in fasting conditions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis enzyme contributes to the production of ketone bodies important components during periods of low glucose availability. HMGCL does not function as part of a multi-protein complex but its action underpins the metabolic switch from glucose to fatty acids and ketone body usage. Proper functioning of this enzyme helps maintain energy balance especially during fasting and extended exercise.\u003cbr\u003e\nPathways\u003cbr\u003e\nHMGCL plays a significant role in the ketogenic pathway linking fatty acid oxidation to ketone body production. It also intersects with leucine degradation pathways reflecting its dual substrate specificity. HMGCL shares links with proteins involved in lipid metabolism such as HMG-CoA synthase which provides the substrate for its enzymatic activity.\u003cbr\u003e\nDefects in HMGCL associate with HMG-CoA lyase deficiency a metabolic disorder hallmarked by hypoketotic hypoglycemia and metabolic acidosis. This condition highlights the enzyme's role in ketogenesis. Additionally its impaired function links to potential exacerbation of seizures due to energy metabolism failure. The enzyme's activity correlates with enzymes like carnitine palmitoyltransferase I which also impact energy metabolism through ketogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850227273897,"sku":"ab197022","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab197022","provider":"Iright","version":"1.0","type":"link"}