{"product_id":"abcam-ab198159","title":"Abcam, ab198159, Recombinant human KDM4B \/ JMJD2B protein","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant human KDM4B \/ JMJD2B protein is a Human Fragment protein, in the 2 to 500 aa range, expressed in Baculovirus infected Sf9 cells, with \u0026gt;68%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;68% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Specific Activity: ≥2.9 pmol\/min\/μg.Assay conditions: 10 μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM α-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.,\u003cbr\u003e\nAccession:O94953,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.55% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843916877993,"sku":"ab198159","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab198159","provider":"Iright","version":"1.0","type":"link"}