{"product_id":"abcam-ab199221","title":"Abcam, ab199221, Alexa Fluor® 488 Anti-RUNX1 \/ AML1 + RUNX3 + RUNX2 antibody [EPR3099]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Recombinant Monoclonal RUNX1 \/ AML1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC\/IF and reacts with Human samples. Cited in 3 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR3099,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nConjugation:Alexa Fluor® 488,\u003cbr\u003e\nExcitation\/Emission:Ex: 495nm, Em: 519nm,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nAlexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle, Store in the dark\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRUNX1 also known as AML1 along with RUNX2 and RUNX3 form a family of transcription factors that play a critical role in gene regulation. These proteins recognize specific DNA sequences through their Runt domain facilitating DNA binding and transcriptional activation or repression. RUNX1 is extensively studied having a mass of approximately 48 kDa. They are expressed in various tissues including hematopoietic cells osteoblasts and the central nervous system. RUNX proteins regulate genes involved in cell differentiation and proliferation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRUNX1 RUNX2 and RUNX3 control processes in development and differentiation. They often function as part of a transcriptional complex with CBFβ enhancing DNA binding and transcriptional activity. RUNX1 is vital in hematopoiesis guiding the formation of blood cells. RUNX2 predominantly influences bone development by regulating osteoblast differentiation while RUNX3 impacts neural development and T-cell maturation. These proteins collectively integrate signals to ensure proper cell lineage decisions.\u003cbr\u003e\nPathways\u003cbr\u003e\nRUNX proteins participate in essential cellular pathways like the Wnt and TGF-β signaling pathways. The Wnt pathway involves interaction with β-catenin affecting cell fate and development processes. RUNX2 plays a significant role here by contributing to bone formation. The TGF-β pathway influences cell growth and differentiation by interacting with SMAD proteins primarily through RUNX3 which modulates immune responses and tumorigenesis. These pathways highlight RUNX proteins' critical integration in signaling networks.\u003cbr\u003e\nRUNX1 mutations associate with various hematological malignancies notably acute myeloid leukemia (AML). RUNX1 mutations disrupt normal hematopoiesis leading to uncontrolled cell proliferation. RUNX2's dysregulation relates to cleidocranial dysplasia a skeletal disorder characterized by delayed bone development. Both RUNX1 and RUNX2 link to similar signaling pathways such as the Wnt pathway implicating them in these diseases' pathogenesis. Understanding RUNX proteins' role can lead to better diagnostic and therapeutic approaches for these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46852609671337,"sku":"ab199221","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab199221","provider":"Iright","version":"1.0","type":"link"}