{"product_id":"abcam-ab199768","title":"Abcam, ab199768, Anti-Alpha Dystroglycan antibody [45-3]","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal DAG1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 5 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:45-3,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThis product has switched from a hybridoma to recombinant production method on 9th September 2020.\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha dystroglycan (alpha-DG) a subunit of the dystroglycan complex acts mechanically as an important cell surface receptor. This protein binds extracellular matrix components like laminin agrin and perlecan facilitating cell-matrix interactions. Alpha-DG has a molecular weight of approximately 156 kDa and comes from post-translational modification of a precursor protein. Researchers frequently use the terms \"alpha\" and \"DG\" when referencing this target. This protein shows wide expression across various tissues including skeletal cardiac and smooth muscle as well as in the brain and peripheral nerves.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlpha-DG performs roles critical for stabilizing tissue architecture by connecting the actin cytoskeleton to the extracellular matrix. As a part of the dystroglycan complex alpha-DG associates with beta-dystroglycan contributing to the linkage between dystrophin and the extracellular components. It ensures structural integrity and signal transduction that are necessary for tissue maintenance and function. Disrupted interactions can lead to compromised tissue stability which reflects its importance in cellular contexts.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlpha-DG integrates into the dystrophin-glycoprotein complex (DGC) a significant component within the mechanotransduction and cellular signaling pathways. The complex notably participates in the PI3K\/AKT signaling pathway important for survival and metabolism along with interactions involving integrins. Through its role in these pathways alpha-DG engages with proteins like dystrophin and sarcoglycans facilitating proper signaling and structural support which is important for normal muscle function.\u003cbr\u003e\nAlpha-DG connects closely with dystroglycanopathies which involve a group of muscular dystrophies like congenital muscular dystrophy. These conditions result from improper glycosylation of α-DG affecting its binding ability and compromising muscular integrity. Alpha-DG also relates to conditions such as cancer where altered glycosylation patterns may impact tumor progression and cell invasion. In these contexts alpha-DG interacts with beta-dystroglycan indicating that both defects in glycosylation and associated proteins contribute to disease mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850328527017,"sku":"ab199768","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab199768","provider":"Iright","version":"1.0","type":"link"}