{"product_id":"abcam-ab200040","title":"Abcam, ab200040, Anti-HAND2 antibody [EPR19451]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nAnti-HAND2 antibody [EPR19451] (ab200040) is a rabbit monoclonal antibody detecting HAND2 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - Biophysical QC for unrivalled batch-batch consistency - Over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR19451,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IP, ICC\/IF, IHC-P, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-HAND2 antibody [EPR19451] (ab200040) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of HAND2?\u003cbr\u003e\nAnti-HAND2 [EPR19451] (ab200040) specifically detects a band for HAND2 (UniProt: Q61039) at a molecular weight of 24kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-HAND2 [EPR19451] (ab200040) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR19451] also available for your convenience: ab200040, Carrier free -\u003cbr\u003e\nab240353\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nHAND2 also known as Heart and Neural Crest Derivatives Expressed 2 is a transcription factor weighing approximately 22 kDa. This protein plays an important role in regulating the expression of genes necessary for specific cell differentiation processes. HAND2 is widely expressed in cardiac and neural tissues with a particular emphasis on the developing heart and nervous system. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which are known for their function in binding to E-box sequences in DNA and controlling gene expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHAND2 influences cardiac morphogenesis and contributes to neural crest cell migration. It engages in complex interactions with other transcription factors to modulate the expression of genes involved in these processes. HAND2 operates as part of a transcriptional complex often forming heterodimers with other bHLH proteins like E47 and HEB which enhances its DNA-binding capability and specificity. This interaction is essential for the activation of downstream targets that control cellular differentiation and proliferation in cardiac and neural development.\u003cbr\u003e\nPathways\u003cbr\u003e\nHAND2 is an important participant in both heart development and neural crest cell signaling. It integrates signals in the BMP (Bone Morphogenetic Protein) pathway and partners with proteins such as NKX2-5 and GATA4 which are critical for orchestrating cardiac gene expression. HAND2 also plays a significant role in the WNT signaling pathway contributing to the proper guidance and differentiation of neural crest derivatives.\u003cbr\u003e\nHAND2 mutations or dysregulation have links to congenital heart defects and neural crest-related disorders. In congenital heart defects altered HAND2 function impacts the expression levels of NKX2-5 and GATA4 leading to improper heart formation. Additionally HAND2's involvement in neural crest cell development associates its dysregulation with diseases such as Hirschsprung's disease. Pathological changes in HAND2 activity potentially disrupt the normal neural crest migration patterns contributing to these developmental anomalies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844107391145,"sku":"ab200040","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab200040","provider":"Iright","version":"1.0","type":"link"}