{"product_id":"abcam-ab202296","title":"Abcam, ab202296, Alexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368]","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-alpha smooth muscle Actin antibody [EPR5368] - Alexa Fluor® 647 conjugated (ab202296) is a rabbit recombinant monoclonal antibody detecting alpha smooth muscle Actin in  ICC\/IF . Suitable for  Human . - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR5368,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nConjugation:Alexa Fluor® 647,\u003cbr\u003e\nExcitation\/Emission:Ex: 650nm, Em: 665nm,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAlexa Fluor® 647 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202296) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 647 (ab202296) has over 5 independent reviews from customers.\u003cbr\u003e\nRelated products\u003cbr\u003e\nAntibody clone EPR5368 is also available pre-conjugated to a variety of labels for your convenience – Anti-alpha smooth muscle Actin Alexa Fluor® 647 [EPR5368] (ab202296).\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR5368] also available for your convenience:\u003cbr\u003e\nab124964\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab202295\u003cbr\u003e\n, Alexa Fluor® 647 - ab202296, Alexa Fluor® 555 -\u003cbr\u003e\nab202509\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab202510\u003cbr\u003e\n, PE -\u003cbr\u003e\nab208844\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab220795\u003cbr\u003e\n, APC -\u003cbr\u003e\nab225143\u003cbr\u003e\n, ab284414, Alexa Fluor® 790 - ab309335,\u003cbr\u003e\nab320058\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab320059\u003cbr\u003e\nab321866\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab322917\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nAlexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle, Store in the dark\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAlpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA\/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.\u003cbr\u003e\nAlpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844228108457,"sku":"ab202296","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab202296","provider":"Iright","version":"1.0","type":"link"}