{"product_id":"abcam-ab202405","title":"Abcam, ab202405, Human Prekallikrein 1B ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Prekallikrein 1B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Prekallikrein 1B with a sensitivity of 154 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 154 pg\/mL,\u003cbr\u003e\nRange:0.47 - 30 ng\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Prekallikrein 1B ELISA Kit ab202405 is a rapid single-wash 90-min Sandwich ELISA to measure Human Prekallikrein 1B in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, serum. This SimpleStep sensitivity is 154 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman Prekallikrein 1B SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman Prekallikrein 1B ELISA Kit ab202405 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nPrekallikrein 1B (KLKB1) is a 88 kDa serine protease involved in blood coagulation, kinin generation, fibrinolysis and inflammation. Prekallikrein 1B is synthesized in the liver and circulates in the blood stream as a free zymogen or in a complex with high molecular weight kininogen. Prekallikrein 1B has a signal peptide (aa1-19) and a pro form (aa20-638). It is composed of a heavy chain and a light chain which are cleaved upon activation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe protein Plasma Kallikrein 1B also known as KLKB1 functions mechanically as a serine protease involved in the kinin-kallikrein system. It is pivotal for initiating pathways relating to inflammation and blood pressure regulation. Plasma Kallikrein 1B is active in plasma with a molecular mass around 88 kDa. It is predominantly expressed in endothelial cells and liver hepatocytes where its precursor form is activated.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPlasma Kallikrein 1B participates in the cleavage of high molecular weight kininogen (HMWK) to release bradykinin a potent vasodilator. This protein is not typically part of a stable macromolecular complex but interacts transiently with other proteins during its activation and function. Its action facilitates increased vascular permeability which plays a role in inflammatory and coagulation responses.\u003cbr\u003e\nPathways\u003cbr\u003e\nPlasma Kallikrein 1B mainly operates within the kallikrein-kinin system and the intrinsic coagulation pathway. It activates Factor XII (Hageman factor) which serves as an upstream regulator in the coagulation cascade. Additionally it connects to other proteins like prekallikrein and high molecular weight kininogen in these pathways coordinating responses in inflammation and blood pressure control.\u003cbr\u003e\nPlasma Kallikrein 1B associates significantly with hereditary angioedema and diabetic retinopathy. Elevated activity or dysregulation of this protein contributes to excessive inflammatory responses seen in these conditions. It also interacts with C1 inhibitor proteins which regulate its activity under normal physiological conditions. This relationship emphasizes its role in maintaining balance within the kinin-kallikrein and related pathways which when disrupted can lead to pathophysiological conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843625963689,"sku":"ab202405","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab202405","provider":"Iright","version":"1.0","type":"link"}