{"product_id":"abcam-ab202417","title":"Abcam, ab202417, Anti-iNOS+nNOS antibody [EP16634]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal iNOS antibody. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Mouse, Mouse, Human, Recombinant full length protein - Human samples. Cited in 4 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP16634,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:I-ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:We were unable to detect a positive signal in inflammation‑induced THP‑1 cells. According to published studies (PMID:33553532, PMID: 24477906), human macrophages produce very little detectable iNOS and NO in response to inflammatory stimuli due to epigenetic regulation around the NOS2 transcription start site.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe iNOS and nNOS also called inducible nitric oxide synthase and neuronal nitric oxide synthase are enzymes that produce nitric oxide (NO) a signaling molecule involved in various physiological processes. iNOS and nNOS differ in their expression and regulation. iNOS is found in various tissues and is induced under inflammatory conditions while nNOS is constitutively expressed in neurons. The molecular weights of iNOS and nNOS are approximately 131 kDa and 160 kDa respectively. Both isoforms exist as homodimers facilitating the conversion of L-arginine to L-citrulline and nitric oxide.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNitric oxide produced by these synthases acts as a neurotransmitter and an immune mediator. In neurons nNOS-derived NO modulates synaptic plasticity and cognitive function significantly affecting processes such as memory and learning. iNOS-derived NO has roles in host defense and inflammation. During immune responses iNOS expression increases and produces NO to help in pathogen destruction. Both enzymes are part of multi-protein complexes that include cofactors like NADPH FAD FMN and tetrahydrobiopterin all necessary for catalytic activity.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe nitric oxide produced by iNOS and nNOS participates in the nitric oxide signaling pathway and the cyclic GMP (cGMP) pathway. Nitric oxide activates soluble guanylate cyclase in the cGMP pathway which in turn regulates various physiological processes including vasodilation. nNOS is related to synaptophysin through neuronal pathways affecting neurotransmitter release and neuronal excitability. iNOS is associated with cytokines like TNF-α and IFN-γ in inflammatory signaling pathways having roles in cell death and immune signaling.\u003cbr\u003e\nNitric oxide dysregulation by iNOS and nNOS impacts numerous conditions including neurodegenerative diseases and chronic inflammation. In neurodegenerative diseases like Alzheimer's excessive NO production by nNOS can lead to neurotoxicity. Inflammatory disorders such as rheumatoid arthritis involve increased iNOS expression resulting in high levels of nitric oxide and consequent tissue damage. Both iNOS and nNOS through NO production interact with proteins such as amyloid-beta in Alzheimer’s and with various cytokines in inflammatory disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844231024809,"sku":"ab202417","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab202417","provider":"Iright","version":"1.0","type":"link"}