{"product_id":"abcam-ab204131","title":"Abcam, ab204131, Anti-PFKM + PFKP antibody [EPR17314]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal PFKP antibody. Suitable for IP, WB, ICC\/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 21 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR17314,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IP, WB, Flow Cyt (Intra), ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPFKM and PFKP are key enzymes involved in glycolysis. These targets are commonly referred to as phosphofructokinase muscle (PFKM) and phosphofructokinase platelet (PFKP). PFKM has a molecular mass of approximately 85 kDa while PFKP is slightly larger at around 84 kDa. PFKM is expressed mainly in muscle tissues whereas PFKP is found in platelets and other tissues like the brain. These enzymes catalyze the conversion of fructose-6-phosphate to fructose-16-bisphosphate using ATP an important regulatory step in glycolysis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPFKM and PFKP play significant roles in energy metabolism. They are part of the phosphofructokinase enzyme complex which regulates glycolytic flux. PFKM is important for muscle energy production during high-intensity exercises where anaerobic respiration predominates. PFKP on the other hand manages glycolytic rates in tissues like brain and platelets which have unique energy demands. The activity of these enzymes is controlled by various metabolites reflecting cellular energy status.\u003cbr\u003e\nPathways\u003cbr\u003e\nPFKM and PFKP are central components of the glycolysis pathway a critical energy-yielding process. Glycolysis not only provides ATP but also intermediates for other pathways like the Krebs cycle. Both PFKM and PFKP interact with enzymes like aldolase and pyruvate kinase coordinating energy production. Their regulation ensures proper adaptation to varying energy requirements across different cell types and tissues.\u003cbr\u003e\nPFKM mutations associate with glycogen storage disease type VII (Tarui's disease) where affected individuals suffer from exercise intolerance and muscle cramps. This connection highlights the importance of PFKM in muscle energy production. PFKP has been linked to cancer metabolism as it is involved in adapting glycolysis rates in tumors often called the Warburg effect. Dysregulation of these enzymes can alter metabolic processes interacting with proteins like lactate dehydrogenase contributing to disease progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850244182185,"sku":"ab204131","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab204131","provider":"Iright","version":"1.0","type":"link"}