{"product_id":"abcam-ab206995","title":"Abcam, ab206995, Microsome isolation kit","description":"\u003cp\u003eSize: 50Test\u003cbr\u003e\nMicrosome Isolation Kit (ab206995) provides a convenient and fast way to isolate microsomal fractions from animal tissues. Suitable for downstream applications e.g. assessing CYP-mediated drug metabolism, xenobiotic biotransformation, and protein profiling of microsomal membrane proteins by SDS-PAGE and western blot. - Designed for isolation of microsomes from up to 400 mg soft tissue or 400 µL pelleted cultured cells.\u003cbr\u003e\nKey facts\u003cbr\u003e\nAssay time:45m,\u003cbr\u003e\nSample types:Tissue, Suspension cells\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMicrosome Isolation Kit (ab206995) provides a convenient and fast way to isolate microsomal fractions from animal tissues for downstream applications such as assessing CYP-mediated drug metabolism and xenobiotic biotransformation, and protein profiling of microsomal membrane proteins by SDS-PAGE and western blotting.\u003cbr\u003e\nThe microsome isolation kit enables preparation of active microsomes, without the need for ultracentrifugation or sucrose gradient fractionation. The kit contains sufficient reagents for 50 isolation procedures, yielding microsomes from roughly 25 grams of tissue or cultured cells.\u003cbr\u003e\nMicrosome isolation protocol summary:\u003cbr\u003e\n- place tissue \/ washed cells in chilled Dounce homogenizer with homogenization buffer\u003cbr\u003e\n- homogenize on ice and suspend in buffer\u003cbr\u003e\n- vortex for 30 s and incubate on ice for 1 min\u003cbr\u003e\n- spin at 10,000 g for 15 min\u003cbr\u003e\n- discard floating lipid layer\u003cbr\u003e\n- spin at 20,000 g for 20 min\u003cbr\u003e\n- retain pellet\u003cbr\u003e\n- wash pellet gently with buffer\u003cbr\u003e\n- resuspend\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K249 Microsome Isolation Kit. K249-50 is the same size as the 50 test size of ab206995.\u003cbr\u003e\nMicrosomes are spherical vesicle-like structures formed from membrane fragments following homogenization and fractionation of eukaryotic cells. The microsomal subcellular fraction is prepared by differential centrifugation and consists primarily of membranes derived from the endoplasmic reticulum (ER) and Golgi apparatus. Microsomes isolated from liver tissue are used extensively in pharmaceutical development, toxicology and environmental science to study the metabolism of drugs, organic pollutants and other xenobiotic compounds by the cytochrome P450 monooxidase (CYP) enzyme superfamily.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847598887081,"sku":"ab206995","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab206995","provider":"Iright","version":"1.0","type":"link"}