{"product_id":"abcam-ab207201","title":"Abcam, ab207201, Total CREB Transcription Factor Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 1 x 96Tests \/ 5 x 96Tests\u003cbr\u003e\nTotal CREB Transcription Factor Assay Kit (Colorimetric) (ab207201) is a high throughput assay to quantify total CREB in nuclear extracts.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Nuclear Extracts,\u003cbr\u003e\nReacts with:Mouse, Rat, Human, Monkey,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nSensitivity:\u0026lt; 500 ng\/well,\u003cbr\u003e\nAssay time:3h 30m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nTotal CREB Transcription Factor Assay Kit (Colorimetric) (ab207201) is a high throughput assay to quantify total CREB in nuclear extracts. This assay combines a quick ELISA format with a sensitive and specific non-radioactive assay for transcription factor binding.\u003cbr\u003e\nA specific double stranded DNA sequence containing the total CREB consensus binding site (5’ –TGACGTCA– 3’) has been immobilized onto a 96-well plate. Total CREB present in the nuclear extract specifically binds to the oligonucleotide. Total CREB is detected by a primary antibody that recognizes an epitope of total CREB accessible only when the protein is bound to its target DNA. An HRP-conjugated secondary antibody provides sensitive colorimetric readout that at OD 450 nm. This product detects total CREB in human, mouse, rat and monkey samples.\u003cbr\u003e\nKey performance and benefits:\u003cbr\u003e\nAssay time: 3.5 hours (cell extracts preparation not included).\u003cbr\u003e\nDetection limit: \u0026lt; 0.5 µg nuclear extract\/well.\u003cbr\u003e\nDetection range: 0.3 – 10 µg nuclear extract\/well.\u003cbr\u003e\nCREB (Cyclic AMP Response Element-Binding protein) is a member of a large family of structurally related transcription factors that includes AFT1-4, c-Fos, c-Jun, c-Myc and C\/EBP. The members of this family, named bZIP, share a dimerization domain with a leucine zipper motif and a DNA-binding domain rich in basic residues (lysines and arginines). CREB proteins specifically recognize the cAMP-responsive element promoter site.\u003cbr\u003e\nAlternative splicing of the CREB gene yields several forms of CREB protein. The three most abundant forms are CREBα (341 aa), also called CREB1, CREBΔ (327 aa) and CREBβ (301 and 387 aa), which are present in human, rat and mouse tissues. Two other gene products highly homologous to CREB-1 have been characterized: activating transcription factor 1 (ATF-1) and cAMP response element modulator (CREM).\u003cbr\u003e\nThe CREB family members bind to the CRE promoter site as homo- and heterodimers. The ratio between these homo- and heterodimers, which depends on the cell type, regulates the CREB transcriptional activity as the homodimers have a longer half-life than CREB\/ATF-1 heterodimers.\u003cbr\u003e\nThe CREB proteins activate transcription of target genes in response to a diverse array of stimuli, such as peptide hormones, growth factors and neuronal activity. Activation of CREB is mediated by a variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs) and Ca\u003cbr\u003e\n\/calmodulin-dependent protein kinases (CaMKs) that phosphorylate CREB at the Ser-133 residue. Phosphorylation of Ser 133 is required for CREB-mediated transcription but not for dimerization and DNA-binding activity. Phosphorylation does, however, increase CREB's affinity for its promoter site. Upon cell stimulation with forskolin, an activator of adenylyl cyclase, the kinases are activated by cAMP production and translocate to the nucleus where they phosphorylate CREB at Ser 133. Therefore, CREB is almost exclusively nuclear in both unstimulated and stimulated cells. A cofactor, CREB-binding protein (CPB), specifically binds to phosphorylated CREB to enhance transcriptional activity.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCAMP response element-binding protein better known as CREB functions as a transcription factor that regulates gene expression. CREB protein operates by binding to specific DNA sequences called cAMP response elements in the promoters of target genes leading to increased or altered transcription. The protein has a molecular weight around 43 kDa. Expressed broadly in many tissues CREB's activity is particularly high in the brain where it plays key roles in neuronal functions. Researchers also refer to phospho-CREB when discussing the activated form of this protein often analyzed through methods like CREB Western blot.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCREB contributes significantly to cellular processes such as proliferation differentiation and survival through transcriptional regulation. CREB does not function alone; it frequently associates with other proteins forming complexes to exert its regulatory roles. Additionally CREB influences the development of memory and learning in neurons highlighting its extensive involvement in neurophysiological processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nCREB plays a substantial role in the cAMP signaling pathway as well as the mitogen-activated protein kinase (MAPK) pathway. Through its interaction with kinases and other signaling proteins CREB influences numerous downstream targets affecting cellular responses to external stimuli. Notably protein kinase A (PKA) activates CREB by phosphorylation facilitating its role in various signal transduction pathways and linking it to downstream transcriptional events.\u003cbr\u003e\nResearch links CREB to neurological disorders and some forms of cancer. Aberrant CREB activity associates with neurodegenerative diseases like Alzheimer’s where dysregulated gene expression impacts neuronal viability and function. Furthermore in cancer CREB's role in cell proliferation and survival ties it to oncogenes such as BCL2 where abnormal CREB function can promote tumorigenesis. Understanding these connections provides valuable insights for therapeutic strategies against such diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855613612201,"sku":"ab207201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab207201","provider":"Iright","version":"1.0","type":"link"}