{"product_id":"abcam-ab207924","title":"Abcam, ab207924, Pig Lipocalin-2 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPig Lipocalin-2 ELISA Kit is a sandwich ELISA designed to quantify Pig Lipocalin-2 with a sensitivity of 1 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 4 - 400 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Urine, Plasma, Serum,\u003cbr\u003e\nReacts with:Pig,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1 pg\/mL,\u003cbr\u003e\nRange:4 - 400 pg\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPig Lipocalin-2 ELISA Kit ab207924 is a sandwich ELISA to measure Pig Lipocalin-2 in serum, plasma, urine with a sensitivity of 1 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe assay is a sandwich ELISA performed in microwells coated with a mouse monoclonal antibody against pig NGAL. Bound NGAL is detected with another mouse monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nPig Lipocalin-2 ELISA Kit ab207924 protocol summary\u003cbr\u003e\n1. Add standards, samples and any controls to appropriate wells\u003cbr\u003e\n2. Wash\u003cbr\u003e\nAdd Biotinylated monoclonal detection antibody and incubate\u003cbr\u003e\n4. Wash.\u003cbr\u003e\n5. Add HRP-conjugated streptavidin.\u003cbr\u003e\n5. Wash\u003cbr\u003e\n6. Add TMB\u003cbr\u003e\n7. Add Stop Solution and read at 450 nm\u003cbr\u003e\nNGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are small secreted proteins characterized by their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, to bind to specific cell-surface receptors, and to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGAL's ability to bind bacterial siderophores. As NGAL was first reported as oncogene 24p3 in the mouse, this name is still occasionally used to identify NGAL in other species.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843644444841,"sku":"ab207924","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab207924","provider":"Iright","version":"1.0","type":"link"}