{"product_id":"abcam-ab208297","title":"Abcam, ab208297, Recombinant E. coli Thermosensitive gluconokinase protein","description":"\u003cp\u003eSize: 20µg\u003cbr\u003e\nRecombinant E. coli Thermosensitive gluconokinase protein is a Escherichia coli K-12 Full Length protein, in the 1 to 187 aa range, expressed in Escherichia coli, with \u0026gt;90%, suitable for SDS-PAGE, FuncS, Mass Spec.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:FuncS, SDS-PAGE, Mass SpecSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Specific activity is \u0026gt; 80 units\/mg, in which one unit will convert 1.0 umole of D-gluconate to 6-phospho-D-gluconate per minute at pH 8.0 at 37C.,\u003cbr\u003e\nAccession:P39208,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Escherichia coli K-12,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThermosensitive gluconokinase known in some studies as GlcK is a kinase enzyme involved in glucose metabolism. It phosphorylates gluconate to 6-phosphogluconate playing an important role in energy conversion processes. The enzyme typically weighs around 40 kDa based on various studies. It is usually expressed in bacterial and some fungal species adjusting its activity in response to environmental temperature changes. Researchers often examine GlcK to understand its thermosensitivity given its unique ability to change kinetic properties with temperature fluctuations.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe enzyme plays an important role in gluconate utilization cycles. The phosphorylation of gluconate to 6-phosphogluconate by thermosensitive gluconokinase is significant for the pentose phosphate pathway where it provides necessary substrates for nucleotide synthesis and antioxidant defense mechanisms. It can function independently or as part of a larger enzymatic complex within the cell depending on the organism and environmental conditions. This flexibility allows bacteria and fungi to adapt their metabolic processes for different surroundings.\u003cbr\u003e\nPathways\u003cbr\u003e\nThermosensitive gluconokinase takes part in the oxidative branch of the pentose phosphate pathway. This pathway not only creates ribose-5-phosphate for nucleotide synthesis but also produces NADPH important for cellular redox reactions. Within this pathway it is often associated with other enzymes like glucose-6-phosphate dehydrogenase. This association allows GlcK to coordinate effectively and maximize metabolic efficiency adapting to cellular needs and extracellular conditions.\u003cbr\u003e\nResearchers have yet to establish strong links between thermosensitive gluconokinase and specific human diseases. However its bacterial counterparts are of interest in understanding metabolic regulation in infectious bacteria which could lead to developing novel antibiotics. In fungi the enzyme's efficiency in glucose metabolism can make it a potential target for antifungal drug development particularly concerning pathogens like Candida species. Connections with proteins involved in metabolic control could reveal new intervention points for drug targeting in both bacterial infections and fungal diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843994243241,"sku":"ab208297","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab208297","provider":"Iright","version":"1.0","type":"link"}