{"product_id":"abcam-ab208774","title":"Abcam, ab208774, Anti-Pro Caspase-8 antibody [EPR162] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal Caspase-8 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR162,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Theoretically, this antibody should recognize p55\/54, p43\/41 and p18. However, in our internal tests on parent antibody ab108333, it only recognized pro caspase 8. We tested it side by side with ab32397. Both antibodies are KO validated, and ab32397 recognizes pro-caspase 8 and cleavage caspase 8.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab208774 is the carrier-free version of\u003cbr\u003e\nab108333\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPro Caspase-8 also known as CASP8 is an inactive precursor of caspase-8 an important enzyme in the apoptosis and necroptosis pathways. This protein has a molecular mass of approximately 55 kDa. Caspase-8 expression occurs in various tissues with higher prevalence in immune cells including T lymphocytes and natural killer cells. Researchers often use techniques like western blot for caspase 8 detection particularly when analyzing its activation state.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPro caspase-8 plays a significant role in the regulation of apoptosis a programmed cell death process. It is initially synthesized in an inactive form and requires activation by cleavage to become functional. Pro caspase-8 integrates into the death-inducing signaling complex (DISC) following specific triggers such as Fas receptor engagement or TNF receptor activation indicating its role in response to external apoptotic signals. Its activation marks the initiation of a cascade that results in apoptosis ensuring controlled cell removal in multicellular organisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nPro caspase-8 functions within the extrinsic apoptosis pathway where it serves as an initiator caspase. Alongside other proteins like Fas-associated protein with death domain (FADD) caspase-8 forms a critical part of the apoptotic signaling network. Another important pathway involving pro caspase-8 is necroptosis an alternative form of programmed cell death where it can inhibit the function of receptor-interacting protein kinase 1 (RIPK1) and RIPK3 under certain conditions. The regulation of these pathways highlights pro caspase-8's central role in determining cell fate under stress or damage.\u003cbr\u003e\nAberrations in pro caspase-8 activity link to various conditions such as cancer and autoimmune diseases. In cancers insufficient caspase-8 activation results in the evasion of apoptosis allowing uncontrolled cell proliferation. Meanwhile excessive or improper activation associates with autoimmune disorders where unregulated apoptosis leads to immune system imbalance. Pro caspase-8 connects with proteins like FLICE-like inhibitory protein (c-FLIP) in cancer where it influences the apoptotic threshold and contributes to the pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851017408681,"sku":"ab208774","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab208774","provider":"Iright","version":"1.0","type":"link"}