{"product_id":"abcam-ab209665","title":"Abcam, ab209665, Anti-Brachyury \/ Bry antibody [EPR18113]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-Brachyury \/ Bry antibody [EPR18113] (ab209665) is a rabbit monoclonal antibody detecting Brachyury \/ Bry in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC- Wmt, ICC\/IF . Suitable for  Human, Mouse, Rat . - Clone EPR18113 is the most cited clone to Brachyury \/ Bry - Biophysical QC for unrivalled batch-batch consistency - Over 30 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR18113,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IHC-P, IP, Flow Cyt (Intra), WB, IHC - Wmt, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-Brachyury \/ Bry antibody [EPR18113] (ab209665) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC\/IF, IHC - Wmt, IHC-P, IP, WB in human, mouse, rat samples.\u003cbr\u003e\nAnti-Brachyury \/ Bry antibody [EPR18113] (ab209665) has been cited over 38 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-Brachyury \/ Bry antibody [EPR18113] (ab209665) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nAnti-Brachyury \/ Bry antibody [EPR18113] (ab209665) has 9 independent reviews from customers.\u003cbr\u003e\nAnti-Brachyury \/ Bry antibody [EPR18113] (ab209665) specifically detects Brachyury \/ Bry (UniProt ID: O15178; Molecular weight: 47kDa) and is sold in 100 µL and 1 mL selling sizes.\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone EPR18113 -\u003cbr\u003e\nab236023\u003cbr\u003e\nAntibody clone EPR18113 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 555 (\u003cbr\u003e\nab314674\u003cbr\u003e\nab314675\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Brachyury protein also known as Bry or T acts as a transcription factor and belongs to the T-box family of genes. It consists of about 435 amino acids giving it an estimated mass of around 47 kDa. Brachyury plays a critical role in the development of the posterior mesoderm and nucleus pulposus during embryogenesis. It is strongly expressed in the notochord mesoderm and some tumor tissues. The protein's presence can be detected using specific assays such as Brachyury IHC (immunohistochemistry) and Brachyury stain which help identify Brachyury marker expression in both normal and pathological states.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe Brachyury protein influences the morphogenetic movement in mesoderm development. It directly interacts with specific DNA sequences to regulate the transcription of target genes. Brachyury often operates as part of a larger transcriptional complex that manages the differentiation and migration of cells during embryogenesis. The gene's expression indicates mesodermal differentiation and is important for cell fate decisions within developing embryos.\u003cbr\u003e\nPathways\u003cbr\u003e\nBrachyury plays a significant role in the Wnt signaling and the TGF-beta pathways. These pathways are essential in controlling cell proliferation and differentiation. Brachyury directly impacts the regulation of these pathways influencing other proteins like beta-catenin in Wnt signaling. This interconnectedness suggests its broader role in cellular communication and development processes potentially affecting gene expression across these pathways.\u003cbr\u003e\nBrachyury has been associated with various cancers such as chordoma and other related tumors. Chordoma specifically involves the notochord where Brachyury marks the disease's presence. Other proteins like E-cadherin interact with Brachyury in tumor progression and metastasis. Understanding Brachyury's function helps in diagnosing and potentially targeting treatment options for these ailments.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844051062953,"sku":"ab209665","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab209665","provider":"Iright","version":"1.0","type":"link"}