{"product_id":"abcam-ab209886","title":"Abcam, ab209886, Recombinant Human Fibronectin type III EDB protein","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nRecombinant Human Fibronectin type III EDB protein is a Human Fragment protein, in the 1244 to 1377 aa range with \u0026gt;90% purity and suitable for SDS-PAGE and Functional studies. The predicted molecular weight of ab209886 protein is 17.6 kDa. - Save time and ensure accurate results - use our fibronectin type III (FN3) EDB protein as a control\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His-T7 tag N-Terminus,\u003cbr\u003e\nApplications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P02751,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 0.32% Tris HCl\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe FN3 EDB ab209886 was expressed in E.coli as inclusion bodies. The final product was refolded using a unique \"temperature shift inclusion body refolding\" technology and chromatographically purified.\u003cbr\u003e\nEnsure the validity of your result using our recombinant human fibronectin type III (FN3) EDB protein\u003cbr\u003e\nab155639\u003cbr\u003e\nas a positive control in SDS-PAGE.\u003cbr\u003e\nab155639\u003cbr\u003e\ncan be used for the following assays:\u003cbr\u003e\n1. In vitro FN-III EDB domain mediated human endothelial cell or cancer stroma cell differentiation \/ migration regulations study with this protein as either soluble factor or coating matrix protein.\u003cbr\u003e\n2. In vitro protein-protein interaction mapping.\u003cbr\u003e\nFunction\u003cbr\u003e\nExtracellular matrix protein fibronectin (FN) plays an important role in cell adhesion. FN contains FN type I, II and III domains. The FN III repeat is generally about 90 amino acid long and to be composed of seven b-strands, forming two antiparallel b-sheets. In the C-terminal of human fibronectin region, there are 16 FN-III repeat domains. FN III domain derived proteins have been demonstrated to bind various proteins with high affinity.\u003cbr\u003e\nThe alternatively-spliced extra-domain B (EDB) of fibronectin represents one of the best characterized markers of angiogenesis. This domain of 91-aminoacids is completely identical between mouse and man, is virtually absent in normal adult tissues (exception made for the endometrium in the proliferative phase and some vessels of the ovaries), but is strongly expressed in most aggressive solid cancer types, with a prominent vascular and\/or stromal pattern of expression.\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843999387817,"sku":"ab209886","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab209886","provider":"Iright","version":"1.0","type":"link"}