{"product_id":"abcam-ab210579","title":"Abcam, ab210579, Mouse Pro-Collagen I alpha 1 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests\u003cbr\u003e\nMouse Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Pro-Collagen I alpha 1 with a sensitivity of 6.7 pg\/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Different formats for different needs: 384-well plate for higher throughput - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 10 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture extracts, Tissue Extracts, Serum,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 6.7 pg\/mL,\u003cbr\u003e\nRange:31.3 - 2000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Pro-Collagen I alpha 1 ELISA Kit ab210579 is a rapid single-wash 90-min ELISA kit to measure Mouse Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, serum and plasma. This SimpleStep ELISA® sensitivity is 6.7 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nMouse Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant mouse Pro-Collagen I alpha 1 protein in serum, plasma, and cell and tissue extract samples only. This kit is not validated for cell culture supernatants.\u003cbr\u003e\nMouse Pro-Collagen I alpha 1 ELISA Kit ab210579 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Mouse Pro-Collagen I alpha 1 ELISA kit ab210579\u003cbr\u003e\nMouse Pro-Collagen I alpha 1 ELISA kit ab210579 has been used to study the effects of ethanol on osteoclast differentiation\u003cbr\u003e\nand the effects of therapeutic candidates for skeletal disorders\u003cbr\u003e\nReferences:\u003cbr\u003e\n-Marin J J Ronis et al. 2022, PMID: 34755883,\u003cbr\u003e\nShinsuke Ohba et al. 2022, PMID: 36475024\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Mouse Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Mouse Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free\u003cbr\u003e\nab241818\u003cbr\u003e\n- Anti-Procollagen Type I antibody [EPR19075-180] - BSA and Azide free (Capture)\u003cbr\u003e\nab242554\u003cbr\u003e\n- Anti-Procollagen Type I antibody [EPR19075-139] - BSA and Azide free (Detector)\u003cbr\u003e\nab242802\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nMouse Pro-Collagen I alpha 1 ELISA kit ab210579 is commonly used to study tissue structure and function. Pro-Collagen I alpha 1 is a component of the extracellular matrix (ECM) and provides structural support to bones.\u003cbr\u003e\nOther ELISA kits to measure the ECM and its role in tissue function include:\u003cbr\u003e\n- Mouse Fibronectin ELISA kit\u003cbr\u003e\nab210967\u003cbr\u003e\n- Human Pro-Collagen I alpha 1 ELISA kit\u003cbr\u003e\nab210966\u003cbr\u003e\nELISA kits to measure bone formation and turnover include:\u003cbr\u003e\n- Mouse Osteocalcin ELISA kit\u003cbr\u003e\nab285236\u003cbr\u003e\n- Human Osteocalcin ELISA kit\u003cbr\u003e\nab270202\u003cbr\u003e\nType I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, mouse Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-151), the mature chain (aa 152-1207), and another propeptide (aa 1208 – 1453). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCollagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCollagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.\u003cbr\u003e\nPathways\u003cbr\u003e\nCollagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.\u003cbr\u003e\nCollagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843535818921,"sku":"ab210579","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab210579","provider":"Iright","version":"1.0","type":"link"}