{"product_id":"abcam-ab211070","title":"Abcam, ab211070, D 2 Hydroxyglutarate Assay Kit (Colorimetric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nD-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG). - Highly specific for D-2-Hydroxyglutarate and does not detect L-2-Hydroxyglutarate\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Tissue Lysate, Urine, Plasma, Serum, Cell Lysate,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:= 10 µM,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nD-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG).\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn the D 2 Hydroxyglutarate assay, D-2-Hydroxyglutarate is oxidized to alpha-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is proportional to the amount of D2HG present in the samples.\u003cbr\u003e\nD 2 Hydroxyglutarate assay protocol summary\u003cbr\u003e\n- Add samples and standards to wells\u003cbr\u003e\n- Add reaction mix\u003cbr\u003e\n- Incubate for 1 hour at 37°C\u003cbr\u003e\n- Analyze with a microplate reader\u003cbr\u003e\nHow other researchers are using ab211070\u003cbr\u003e\nD 2 Hydroxyglutarate Assay Kit (Colorimetric) has been used in a variety of sample types including:\u003cbr\u003e\n- Human glioma samples\u003cbr\u003e\n- Primary human astrocytes\u003cbr\u003e\n- Human Diffuse large B cell lymphoma\u003cbr\u003e\nReferences: 1-Chen X al. 2023, 2-Bredel M et al. 2023, 3-Cai Y et al. 2023\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K213 Biovision D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nIn eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and alpha-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of alpha ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG, D-2-Hydroxyglutarate) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843549974697,"sku":"ab211070","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab211070","provider":"Iright","version":"1.0","type":"link"}