{"product_id":"abcam-ab211156","title":"Abcam, ab211156, Phagocytosis Assay Kit (Zymosan, Colorimetric)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPhagocytosis Assay Kit ab211156 uses a Zymosan substrate to provide a robust system for screening TLR ligands, phagocytosis activators or inhibitors.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Adherent cells,\u003cbr\u003e\nAssay type:Cell-based,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhagocytosis Assay Kit ab211156 uses a Zymosan substrate to provide a robust system for screening TLR ligands, phagocytosis activators or inhibitors.\u003cbr\u003e\nColorimetric phagocytosis assay principle\u003cbr\u003e\nThe phagocytosis assay uses pre-labeled Zymosan particles as a pathogen to trigger phagocytosis. The label on the engulfed Zymosan particles then reacts with a substrate to produce a colorimetric signal that can be detected by absorbance at 405 nm.\u003cbr\u003e\nExternal particles are blocked before the reaction with a blocking reagent, ensuring the signal is directly proportional to the amount of internalized particles.\u003cbr\u003e\nThis format provides a quantitative, high-throughput method to accurately measure phagocytosis, and avoids subjective manual counting of Zymosan particles inside cells.\u003cbr\u003e\nPhagocytosis assay protocol summary\u003cbr\u003e\n- add Zymosan substrate to cell cultures and incubate for 15 min to 2 hrs\u003cbr\u003e\n- remove culture medium, and wash cells\u003cbr\u003e\n- add fixation solution and incubate for 5 min and then wash cells\u003cbr\u003e\n- add blocking reagent and incubate for 60 min and then wash cells\u003cbr\u003e\n- add permeabilization solution and incubate for 5 min and then wash cells\u003cbr\u003e\n- add detection reagent and incubate for 60 min and then wash cells\u003cbr\u003e\n- add detection buffer and incubate for 10 min\u003cbr\u003e\n- add substrate and incubate for 5-20 min\u003cbr\u003e\n- add stop solution and analyze with plate reader\u003cbr\u003e\nGetting the best performance from ab211156\u003cbr\u003e\nThis kit is suitable for adherent phagocytes only.\u003cbr\u003e\nEach 20 test kit provides sufficient quantities to perform 20, 10 or 5 tests in a 96-,48- or 24-well plate respectively. Each 96 tests kit provides sufficient quantities to perform 96, 48 or 24 tests in a 96-, 48- or 24-well plate respectively. Each 5 x 96 tests kit provides sufficient quantities to perform 5 x 96, 48 or 24 tests in a 96-, 48- or 24-well plate respectively.\u003cbr\u003e\nRelated phagocytosis assays\u003cbr\u003e\nPhagocytosis Assay Kit (Green E.coli)\u003cbr\u003e\nab235900\u003cbr\u003e\nPhagocytosis Assay Kit (Red E. coli)\u003cbr\u003e\nab235901\u003cbr\u003e\nPhagocytosis Assay Kit (Green Zymosan)\u003cbr\u003e\nab234053\u003cbr\u003e\nPhagocytosis Assay Kit (Red Zymosan)\u003cbr\u003e\nab234054\u003cbr\u003e\nOther notes\u003cbr\u003e\nIn mammals, phagocytosis by phagocytes (e.g., macrophages, dendritic cells, and neutrophils) is essential for a variety of biological events. Phagocytosis comprises a series of events, starting with the binding and recognition of particles by cell surface receptors, followed by the formation of actin-rich membrane extensions around the particle.\u003cbr\u003e\nZymosan (Saccharomyces cerevisiae) is prepared from yeast cell wall and consists of protein-carbohydrate complexes. Zymosan is a commonly used pathogen in phagocytosis assays.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843637137577,"sku":"ab211156","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab211156","provider":"Iright","version":"1.0","type":"link"}