{"product_id":"abcam-ab213779","title":"Abcam, ab213779, Human Ficolin-3 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Ficolin-3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Ficolin-3 in Human in Cell Culture Media, Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Heparin Plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 20 pg\/mL,\u003cbr\u003e\nRange:0.78 - 50 ng\/mL,\u003cbr\u003e\nAssay time:3h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe Human Ficolin-3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213779) is designed for the quantitative measurement of Human Ficolin-3 in cell culture supernatants, serum and plasma (heparin).\u003cbr\u003e\nThe ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-3 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: Q24-R299) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Ficolin-3 amount of sample captured in plate.\u003cbr\u003e\nFicolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFCN3 also known as ficolin-3 is a multifunctional protein recognized for its role in the immune system. Ficolin-3 is approximately 35 kDa in mass and is mainly expressed in the liver though it can also be found in various other tissues such as the spleen and lungs. It belongs to the ficolin family of proteins which are involved in innate immunity. The protein features a collagen-like domain and a fibrinogen-like domain facilitating its binding to sugars on microbial surfaces.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFicolin-3 acts as an essential player in the immune response contributing to pathogen recognition and clearance. It forms a complex with other components to activate the complement pathway particularly the lectin pathway. Ficolin-3 binds to carbohydrate patterns on the surface of pathogens leading to opsonization and clearance. This activity highlights its function as a defense mechanism against infections and its role in recognizing altered cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nFicolin-3 facilitates the activation of the lectin complement pathway. This pathway is important for innate immune defense and involves several other proteins including mannose-binding lectin (MBL) and MASP-2 which are connected with ficolin-3 in mediating complement activation. The engagement of ficolin-3 in this pathway highlights its importance in identifying and eliminating pathogens effectively.\u003cbr\u003e\nMutations or deficiencies in the function of ficolin-3 are associated with an increased risk of infections and autoimmune diseases such as systemic lupus erythematosus (SLE). The protein's role in immune defense makes it a critical factor in maintaining a balanced immune response. Additionally related proteins like MBL can also influence the progression or predisposition to these conditions further emphasizing the interconnectedness of ficolin-3 within immune regulatory networks.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855672823977,"sku":"ab213779","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab213779","provider":"Iright","version":"1.0","type":"link"}