{"product_id":"abcam-ab214030","title":"Abcam, ab214030, Human IL-8 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nHuman IL-8 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-8 (IL-8) with a sensitivity of 1.8 pg\/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader - Available in different formats to meet various throughput needs, including 384-well plates for higher throughput - Broad sample compatibility – suitable for serum, plasma, cell culture supernatants, and cerebrospinal fluid - Cited in over 30 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.8 pg\/mL,\u003cbr\u003e\nRange:3.91 - 250 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-8 ELISA Kit ab214030 is a rapid single-wash 90-min ELISA kit to measure Human IL-8 in cerebral spinal fluid, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 1.8 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman IL-8 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant human IL-8 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.\u003cbr\u003e\nHuman IL-8 ELISA Kit ab214030 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Human IL-8 ELISA Kit ab214030\u003cbr\u003e\nHuman IL-8 ELISA Kit ab214030 has been used to assess inflammation in different physio-pathological contexts such as traumatic brain injury\u003cbr\u003e\n, pulmonary disease\u003cbr\u003e\n, and osteoarthritis\u003cbr\u003e\nReferences:\u003cbr\u003e\nJindan Gao et al. 2022, PMID: 36247290,\u003cbr\u003e\nRui Zheng et al. 2022, PMID: 35191419,\u003cbr\u003e\nZhi Lv et al. 2021, PMID: 34082824\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human IL-8 ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation\u003cbr\u003e\n- Human\/Monkey IL-8 Antibody Pair - BSA and Azide free\u003cbr\u003e\nab241839\u003cbr\u003e\n- Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture)\u003cbr\u003e\nab242515\u003cbr\u003e\n- Anti-IL-8 antibody [EPR19358-108] - BSA and Azide free (Detector)\u003cbr\u003e\nab242983\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman IL-8 ELISA Kit ab214030 is commonly used to measure IL-8 as a marker of inflammation.\u003cbr\u003e\nOther ELISA kits to measure inflammatory markers include:\u003cbr\u003e\n- Human TNF alpha ELISA kit\u003cbr\u003e\nab181421\u003cbr\u003e\n- Human IL-6 ELISA kit\u003cbr\u003e\nab178013\u003cbr\u003e\n- Human IL-1 beta ELISA kit\u003cbr\u003e\nab214025\u003cbr\u003e\nIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843535130793,"sku":"ab214030","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab214030","provider":"Iright","version":"1.0","type":"link"}