{"product_id":"abcam-ab214282","title":"Abcam, ab214282, Anti-CD20 antibody [EP459Y] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nAnti-CD20 antibody [EP459Y] - BSA and Azide free (ab214282) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting CD20 in  Western Blot, Flow Cytometry, IP, IHC-P, ICC\/IF . Suitable for  Human . - KO validated for confirmed specificity - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP459Y,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, IP, ICC\/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-CD20 antibody [EP459Y] - BSA and Azide free (ab214282) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of CD20?\u003cbr\u003e\nAnti-CD20 [EP459Y] - BSA and Azide free (ab214282) specifically detects a band for CD20 (UniProt: P11836) at a molecular weight of 33kDa.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-CD20 antibody [EP459Y] - BSA and Azide free (ab214282) has been confirmed by Western blot testing in MS4A1 Knockout Raji cell line,\u003cbr\u003e\nab273871\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EP459Y] also available for your convenience:\u003cbr\u003e\nab78237\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab166865\u003cbr\u003e\n, Alexa Fluor® 488 -\u003cbr\u003e\nab198941\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab198943\u003cbr\u003e\n, Carrier free - ab214282,\u003cbr\u003e\nab279298\u003cbr\u003e\nab279299\u003cbr\u003e\nab279300\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab279310\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab279311\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab279312\u003cbr\u003e\n, ab284424, PE -\u003cbr\u003e\nab303031\u003cbr\u003e\n, APC -\u003cbr\u003e\nab303032\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab303033\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308897\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310623\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab312151\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312637\u003cbr\u003e\n, FITC -\u003cbr\u003e\nab322915\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.\u003cbr\u003e\nPathways\u003cbr\u003e\nCD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.\u003cbr\u003e\nCD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851742105769,"sku":"ab214282","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab214282","provider":"Iright","version":"1.0","type":"link"}