{"product_id":"abcam-ab215765","title":"Abcam, ab215765, Anti-Emi1 antibody [EMI1\/1176]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nMouse Monoclonal Emi1 antibody. Suitable for IHC-P, WB, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBXO5 aa 1-250.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EMI1\/1176,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, Protein ArraySee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human FBXO5 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.Q9UKT4\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A\/G, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEmi1 also known as FBXO5 is a regulatory protein involved in cell cycle control with a molecular mass of approximately 48 kDa. It functions by inhibiting the anaphase-promoting complex\/cyclosome (APC\/C) an important E3 ubiquitin ligase that tags proteins for degradation during mitosis. Emi1 is expressed in a variety of tissues including those with actively dividing cells like bone marrow and lymph nodes. The regulatory role of Emi1 ensures the proper timing of mitosis preventing premature progression to anaphase.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEmi1 serves as an essential cell cycle regulator by controlling the activity of the APC\/C therefore maintaining genomic stability during cell division. It acts independently not as part of a larger protein complex ensuring that cyclin B and securin levels remain sufficient to hold the cell in metaphase until all chromosomes are correctly attached to the spindle apparatus. This function prevents chromosomal instability and aneuploidy which can lead to malignancies.\u003cbr\u003e\nPathways\u003cbr\u003e\nEmi1 plays a critical role in the cell cycle regulatory network by interacting with the APC\/C complex. By regulating the degradation of cell cycle proteins Emi1 helps maintain the balance between cell proliferation and apoptosis. The regulatory influence of Emi1 on the APC\/C involves interactions with Cdc20 and Cdh1 two key activators of APC\/C which ensures proper cell cycle progression and checkpoint control.\u003cbr\u003e\nAlterations in Emi1 expression or function can contribute to the development of cancer and other proliferative disorders. Emi1 overexpression is frequently found in many cancers including breast and prostate cancer where it contributes to unchecked cell proliferation. Through its interaction with the APC\/C complex aberrant Emi1 activity can disrupt normal cell cycle control mechanisms leading to tumorigenesis. The protein's role in preventing APC\/C-mediated degradation also makes it a connecting factor to proteins like cyclin B linking Emi1 to processes involved in oncogenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855384006825,"sku":"ab215765","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab215765","provider":"Iright","version":"1.0","type":"link"}