{"product_id":"abcam-ab216064","title":"Abcam, ab216064, Human Pro-Collagen I alpha 1 Matched Antibody Pair Kit","description":"\u003cp\u003eSize: 5 x 96Tests\u003cbr\u003e\nHuman Pro-Collagen I alpha 1 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human Pro-Collagen I alpha 1 by sandwich ELISA method.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nSensitivity:= 8.44 pg\/mL,\u003cbr\u003e\nRange:62.5 - 4000 pg\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Pro-Collagen I alpha 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Pro-Collagen I alpha 1.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP\/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (\u003cbr\u003e\nab210905\u003cbr\u003e\n).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit\u003cbr\u003e\nab210966\u003cbr\u003e\n. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nProcollagen Type 1 also known as Pro-1 collagen is a precursor molecule for collagen Type I which is the most abundant protein in the human body. Its molecular mass is approximately 230 kDa. This protein is synthesized by fibroblasts and is widely expressed in connective tissues including skin tendons and bones. Once synthesized procollagen Type 1 undergoes post-translational modifications and is cleaved by specific enzymes to form mature collagen fibers contributing to the structural integrity of the extracellular matrix.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe synthesis and processing of procollagen Type 1 play a major role in maintaining tissue strength and elasticity. This molecule is not part of a larger complex but undergoes self-assembly to form the rigid triple-helical structure typical of collagen fibers. These fibers are essential for providing mechanical support to tissues and facilitating cell adhesion. Additionally its presence is often required for normal wound healing processes and tissue regeneration.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe secretion and assembly of procollagen Type 1 occur through the secretory pathway. Transforming growth factor-beta (TGF-beta) signaling significantly regulates this process influencing fibrogenesis. This pathway also involves the interaction with proteins such as fibronectin and integrins which are important for aligning collagen fibers within the extracellular matrix. Furthermore procollagen Type 1 engages in the Wnt signaling pathway affecting the arrangement and stability of collagen networks.\u003cbr\u003e\nMutations or dysregulation of procollagen Type 1 synthesis may contribute to conditions such as osteogenesis imperfecta and scleroderma. In osteogenesis imperfecta defective collagen leads to brittle bones due to improper fiber formation. In scleroderma an overproduction of collagen causes skin thickening and organ fibrosis. The altered interaction of procollagen Type 1 with proteins like lysyl oxidase can exacerbate these pathological conditions highlighting the significance of proper collagen processing and assembly for tissue health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843577532585,"sku":"ab216064","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab216064","provider":"Iright","version":"1.0","type":"link"}