{"product_id":"abcam-ab216167","title":"Abcam, ab216167, Human IL-17A ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman IL-17A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IL-17A with a sensitivity of 2.6 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 10 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 2.6 pg\/mL,\u003cbr\u003e\nRange:3.125 - 200 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-17A ELISA Kit ab216167 is a rapid single-wash 90-min Sandwich ELISA to measure Human IL-17A in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2.6 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman IL-17A SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman IL-17A ELISA Kit ab216167 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-IL-17A antibody [EPR19941-88] - BSA and Azide free (Capture)\u003cbr\u003e\nab242855\u003cbr\u003e\n- Anti-IL-17A antibody [EPR19941-89] - BSA and Azide free (Detector)\u003cbr\u003e\nab242855\u003cbr\u003e\nIL-17A is a proinflammatory cytokine that is secreted by activated T cells. It is a disulfide-linked homodimer with both glycosylated and nonglycosylated forms. IL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1\/intracellular adhesion molecule 1 in fibroblasts.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843547058345,"sku":"ab216167","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab216167","provider":"Iright","version":"1.0","type":"link"}