{"product_id":"abcam-ab216263","title":"Abcam, ab216263, Anti-Intron-encoded endonuclease I-SceI antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal Intron-encoded endonuclease I-SceI antibody. Suitable for WB, ICC\/IF and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C SCEI.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Saccharomyces cerevisiae,\u003cbr\u003e\nApplications:ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C SCEI.P03882\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-Whole antiserum, Storage buffer-Preservative: 0.05% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIntron-encoded endonuclease I-SceI also known as endonuclease I-SceI or I-SceI endonuclease is a precise and highly specific endonuclease. It recognizes and cleaves an 18-base pair sequence. This sequence is rare resulting in the enzyme being used as a molecular tool for genome editing. I-SceI is encoded by a group I intron within the mitochondrial DNA of yeast and possesses a molecular mass of approximately 30 kDa. The enzyme expression is primarily observed in eukaryotic organisms often associated with mitochondrial genetic material.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nI-SceI facilitates homologous recombination and DNA repair by introducing double-strand breaks at specific genetic locations. Through this action it participates in gene conversion processes allowing for the correction of genetic sequences. It does not operate as a part of a larger protein complex; rather its function centers on individual action and recognition of its target sites. Biological effectiveness makes it an attractive candidate for genomic studies and manipulations.\u003cbr\u003e\nPathways\u003cbr\u003e\nI-SceI contributes significantly to DNA repair pathways particularly homologous recombination. This pathway is important for maintaining genomic stability. I-SceI cooperates with proteins such as RAD51 which facilitates strand exchange during the DNA repair process. In another pathway I-SceI-induced breaks can spur the activation of the single-strand annealing pathway when homologous recombination is impaired showcasing its versatile role in DNA repair mechanisms.\u003cbr\u003e\nI-SceI's role in DNA repair connects it to cancer research. Impaired DNA repair mechanisms can lead to genomic instability a contributing factor to tumorigenesis. By enabling targeted gene corrections I-SceI may present therapeutic potential in correcting cancer-causing mutations. Additionally its interaction with the RAD51 protein implicates it in conditions where DNA repair is compromised such as certain genetic disorders with defective recombination.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854051692713,"sku":"ab216263","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab216263","provider":"Iright","version":"1.0","type":"link"}