{"product_id":"abcam-ab216945","title":"Abcam, ab216945, Human Agrin ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Agrin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Agrin with a sensitivity of 4.6 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Design your own immunoassay: we also offer the conjugation-ready antibody pair - Cited in over 10 citations\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cerebral Spinal Fluid, Urine, Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 4.6 pg\/mL,\u003cbr\u003e\nRange:28.13 - 1800 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Agrin ELISA Kit ab216945 is a rapid single-wash 90-min Sandwich ELISA to measure Human Agrin in cell culture extracts, cell culture supernatant, CSF, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts, urine. This SimpleStep sensitivity is 4.6 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman Agrin SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman Agrin ELISA Kit ab216945 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-Agrin antibody [EPR20025-20] - BSA and Azide free (Capture)\u003cbr\u003e\nab242996\u003cbr\u003e\n- Anti-Agrin antibody [EPR20025-97] - BSA and Azide free (Detector)\u003cbr\u003e\nab242996\u003cbr\u003e\nAgrin is a heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. Agrin is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. Agrin function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Agrin modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Agrin functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+\/K+-ATPase and evoking depolarization at CNS synapses.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAgrin also known as AGRN is a large heparan sulfate proteoglycan with a molecular mass of approximately 200 kDa. This protein is mainly expressed in the neuromuscular junction and the central nervous system. Agrin has significant roles in synapse formation. It influences the clustering of acetylcholine receptors at the neuromuscular junction which is essential for communication between nerve and muscle cells. This activity is mechanically mediated through interactions with the receptor tyrosine kinase MuSK and LRP4.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe Agrin protein contributes to the stabilization of synaptic structures. Its ability to induce acetylcholine receptor clustering is fundamental for the proper formation and maintenance of neuromuscular synapses. Agrin is not a lone player; it is a part of a larger signaling complex. This complex includes other proteins like MuSK which acts as a receptor playing an essential role in complex signaling pathways that regulate synaptogenesis and synaptic plasticity.\u003cbr\u003e\nPathways\u003cbr\u003e\nMany molecules associate to facilitate communication and synapse integrity via Agrin's activity. Agrin engages primarily with the MAPK\/ERK signaling pathway and the PI3K\/AKT pathway. These pathways are central for cell growth and differentiation in the central nervous system. The relationship with MuSK through these pathways highlights Agrin's importance in orchestrating the synapse assembly and stability.\u003cbr\u003e\nMutations or alterations in Agrin can lead to conditions such as congenital myasthenic syndromes and potentially impact neurodegenerative disorders like amyotrophic lateral sclerosis. In these cases its interaction with proteins like MuSK is affected leading to impaired neuromuscular transmission. Understanding the anti-Agrin antibody's function might enhance methodologies to treat these neuromuscular disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843536244905,"sku":"ab216945","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab216945","provider":"Iright","version":"1.0","type":"link"}