{"product_id":"abcam-ab216947","title":"Abcam, ab216947, Mouse GDF-15 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse GDF-15 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GDF-15 with a sensitivity of 22.2 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 22.2 pg\/mL,\u003cbr\u003e\nRange:62.5 - 4000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse GDF-15 ELISA Kit ab216947 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse GDF-15 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 22.2 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nMouse GDF-15 SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nMouse GDF-15 ELISA Kit ab216947 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-GDF15 antibody [EPR18244-236] - BSA and Azide free (Capture)\u003cbr\u003e\nab242888\u003cbr\u003e\n- Anti-GDF15 antibody [EPR18244-97] - BSA and Azide free (Detector)\u003cbr\u003e\nab242888\u003cbr\u003e\nThe cytokine growth differentiation factor 15 (GDF-15) is one of 15 growth\/differentiation factors (GDF-1 to GDF-15), all of which are members of the BMP family of TGF-beta superfamily proteins. These growth\/differentiation factors are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. Specifically, mouse GDF-15 is synthesized as a 273 amino acid (aa) proprecursor that contains a cleavable 158 aa glycosylated prodomain (aa 31-188) and a 115 aa mature region (aa 189-303). Over aa sequence 189-303, mouse GDF-15 shares 97% and 68% aa identity with rat and human GDF-15, respectively.\u003cbr\u003e\nIn mice, GDF-15 is expressed in a diverse population of activated cell types, including hepatocytes, macrophages, Schwann cells, cardiomyocytes, osteoblasts, adipocytes. It is also expressed in the epithelium of the small intestine and mammary gland. GDF proteins have a critical role during embryonic development, particularly in the skeletal, nervous, and muscular systems.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843557183657,"sku":"ab216947","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab216947","provider":"Iright","version":"1.0","type":"link"}