{"product_id":"abcam-ab218298","title":"Abcam, ab218298, Porcine VEGF-A ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPorcine VEGF-A ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine VEGF-A in Pig in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Pig,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 40 pg\/mL,\u003cbr\u003e\nRange:40 - 10000 pg\/mL,\u003cbr\u003e\nAssay time:4h 45m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe Porcine VEGF-A ELISA kit (ab218298) is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Porcine VEGF-A in serum (Porcine VEGF-A concentration is low in normal serum and plasma samples and may not be detectable in this assay), plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for VEGF-A coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF-A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine VEGF-A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is Measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nVEGF 164 also known as Vascular Endothelial Growth Factor 164 plays an important role in angiogenesis and vascular permeability. A member of the VEGF family it serves as a signal protein that promotes the growth of new blood vessels. Vegf 164 is expressed in various tissues and is specially important in the response to ischemic events and tissue remodeling. The molecular mass of VEGF 164 is approximately 45 kDa making it a protein of moderate size within its family.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nVEGF 164 is essential in cellular processes related to vascular growth and blood vessel formation. It is involved in endothelial cell proliferation migration and survival necessary steps for new blood vessel formation. The protein often functions as part of a larger complex that includes co-receptors like neuropilins enhancing its activity and specificity in various tissues. Overall its role is significant in maintaining the structural integrity of the vasculature.\u003cbr\u003e\nPathways\u003cbr\u003e\nVEGF 164 is integrated into angiogenesis and wound healing pathways. It activates several signaling cascades including the PI3K\/Akt and MAPK pathways which further involve related proteins like Akt1 and MAPK1. By mediating these pathways VEGF 164 ensures adequate nutrient and oxygen supply to growing tissues or responding to injury.\u003cbr\u003e\nVEGF 164 has strong associations with cancer and age-related macular degeneration (AMD). In cancer it contributes to tumor growth and metastasis by aiding in the construction of new blood vessels that supply tumor cells. In AMD VEGF 164 levels can increase leading to abnormal blood vessel growth in the eye damaging vision. The protein interacts with similar function proteins like VEGF receptor 2 which is important in same pathological conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843562557609,"sku":"ab218298","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab218298","provider":"Iright","version":"1.0","type":"link"}