{"product_id":"abcam-ab220813","title":"Abcam, ab220813, Anti-CEBP Beta antibody [E299] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal CEBP Beta antibody. Carrier free. Suitable for IP, WB, ICC\/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 12 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:E299,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:IP, Flow Cyt (Intra), WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody is specific for the three CEBPB isoforms (LAP*, LAP and LIP). According to BLAST analysis, the antibody could cross-react with CEBP epsilon (32, 27 and 14kDa, 82% homology) and CEBP alpha (42kDa, 30kDa, 73% homology) in human, mouse and rat. Please be aware that this has not been confirmed experimentally. However, this could explain the background that could possibly be obtained in WB with this antibody. Please contact our Scientific Support if you have any questions.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab220813 is the carrier-free version of\u003cbr\u003e\nab32358\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCEBP Beta also known as CCAAT\/enhancer binding protein beta or C\/EBPβ is a transcription factor that regulates gene expression in various tissues. It has a molecular mass of approximately 35 kDa. CEBP Beta is expressed in liver adipose tissue and immune cells among others. It binds to DNA at specific CCAAT motifs and regulates transcription of genes involved in immune response metabolism and differentiation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCEBP Beta plays important roles in cellular differentiation proliferation and immune function. CEBP Beta is part of the CEBP family of transcription factors which form homo- or hetero-dimers to function effectively often with other family members like CEBP Alpha. Its activity governs important processes in hematopoiesis and adipogenesis. This transcription factor is therefore essential in the regulation of energy homeostasis and immune response influencing the expression of various cytokines and acute-phase proteins.\u003cbr\u003e\nPathways\u003cbr\u003e\nCEBP Beta integrates signals in key biological pathways such as the JAK-STAT and NF-kB pathways. Activation via cytokine signaling CEBP Beta interacts with STAT proteins to modulate gene expression linked to inflammatory responses. It has a significant role in the NF-kB pathway where it cooperates with Rel proteins to regulate genes involved in immune and stress responses including TNF and IL-1.\u003cbr\u003e\nCEBP Beta is implicated in conditions such as acute myeloid leukemia (AML) and obesity-related complications. Abnormal regulation of CEBP Beta can contribute to leukemogenesis by altering the expression of cell cycle and differentiation genes. Additionally CEBP Beta's role in lipid storage and insulin sensitivity links it to metabolic disorders such as Type 2 diabetes. Interactions with proteins like PPAR gamma in adipose tissues influence these disease processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851596845225,"sku":"ab220813","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab220813","provider":"Iright","version":"1.0","type":"link"}