{"product_id":"abcam-ab222408","title":"Abcam, ab222408, Anti-cleaved N-terminal DFNA5 \/ GSDME antibody [EPR20867-248]","description":"\u003cp\u003eSize: 100µL \/ 1mL\u003cbr\u003e\nRabbit Recombinant Monoclonal DFNA5\/GSDME antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR20867-248,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody may weakly cross react with the full length DFNA5\/GSDME.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe cleaved N-terminal DFNA5 also known as GSDME is a significant player in cellular processes. Upon cleaving it turns into an active fragment that carries out distinct functions. GSDME is an effector of necrotic cell death especially pyroptosis with a molecular mass of around 60 kDa. It resides within the cytoplasm of cells displaying expression in various tissues such as the colon stomach and esophagus. This protein functions as a security mechanism modulating cellular responses to stress and damage.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe cleaved N-terminal fragment of GSDME disrupts the cell membrane using its pore-forming ability. GSDME belongs to the gasdermin family and becomes activated by caspase-3 a well-known executioner of apoptosis. It forms a part of a complex cellular machinery influencing the immune response and cell death. The action of GSDME in the apoptosis or pyroptosis decision is critical since it helps balance cell death pathways and inflammation preventing uncontrolled cell proliferation or excessive inflammation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe cleaved N-terminal GSDME integrates into the intrinsic apoptosis pathway acting downstream of caspase-3 activation. GSDME influences the balance between apoptosis and pyroptosis a critical decision point for cell fate in response to intracellular stress. It works in concert with other gasdermin family proteins such as GSDMD which plays a primary role in pyroptosis. The interaction between these proteins allows cells to select appropriate death pathways under different conditions revealing GSDME’s versatility in cellular functioning.\u003cbr\u003e\nThe function of cleaved N-terminal GSDME links to hearing loss and various cancers. Mutations in GSDME associate with autosomal dominant forms of hearing loss highlighting its role in cochlear function. Furthermore low expression levels of GSDME have connections with cancer cell survival and proliferation. This protein interacts with proteins like caspase-3 in cancer where the cleavage of GSDME may promote pyroptosis over apoptotic pathways offering insights into potential therapeutic targets for tumor management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850213445801,"sku":"ab222408","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab222408","provider":"Iright","version":"1.0","type":"link"}